The morphology was observed and the various stage follicles in ovarian tissue and fertility rate, cleavaged rate, good quality embryos rate, blastocysts rate were analyzed, compared with the groups of non-stimulated and single ovarian stimulated groups.

The cleavage rate and the blastocyst rate of oocytes activated by Eth + 6-DMAP(35.

There was no effect of the three PVP concentrations on cleavage rate after sperm injection,but regular cleavage rate,morulae rate and blastocyst rate were improved with decreasing concentration of PVP,cleavage rate (69.

The results showed:0 h,0 5 h groups took the highest cleavage rate(CR) which were 87 7%、84 7% respectively, comparing with the other groups, the difference is significant(P<0 01); 0 5 h group had the highest blastocyst rate(BR)(67 5%), the difference is signif.