In this study, to develop a vaccine to protect mandarin fish against infection with ISKNV, the immunogenicity of the recombinant major capsid protein(MCP) was tested with several experiments.

To produce human papillomavirus (HPV) type 6 major capsid protein (L1) for screening the sera of condyloma acuminata (CA) patients, HPV 6 L1 protein was produced by recombinant baculovirus expression system and purified with affinity chromatography and was used as antigen to analyse by ELISA the sera of 30 CA patients, 20 blood donors and 10 children for the presence of IgG antibody.

AIM: To express the major capsid protein of human papillomavirus type 16 L1(HPV 16 L1) in E.

Preparation and characterization of monoclonal antibodies against Norovirus capsid protein;

Expression of the fusion protein of dengue virus capsid protein C and staphylococcal nuclease in Escherichia coli;

Expression and self-assembly of dengue virus capsid protein;

Codon Optimization and Expression of Norovirus Capsid Proteins in Insect Cells;

Construction and Characterization of SHIV Carrying HIV-1 Capsid;

Expression of Matrix and Capsid Portein of Bovine Immunodeficiency Virus;

Cloning and expression of the coat protein gene of IHHNV in shrimp and its antiserum preparation;

Site-directed mutagenesis was performed at the codon 15 of the MS2 bacteriophage coat protein gene,which had been cloned to the virus-like particles expression vector containing non-self RNA fragment.

The analysis results of nucleotide sequences of their coat protein genes with those of other 2 TuMV isolates showed that they could be clustered into three groups.