1) Sequence analysis with clone of PCR products
PCR产物克隆测序
2) PCR-Cloning-Sequencing
PCR-克隆序列
1.
To investigate the effects of real-time control on the structure of nitrifying communities of the short-cut nitrogen removal in SBR pilot-scale plant with a working volume of 54 m~3,molecular techniques of Fish,PCR-DGGE and PCR-Cloning-Sequencing were used to analyze ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) with the performance of short-cut nitrogen removal.
为了研究实时控制对短程生物脱氮中试系统内硝化菌群结构组成的影响,在考察系统常温条件下处理城市污水时短程硝化效果的同时,采用FISH、PCR-DGGE和PCR-克隆序列(Cloning Sequencing)分子生物学方法对SBR中试系统(有效容积为54 m~3)硝化菌群中的氨氧化菌(AOB)和亚硝酸氧化菌(NOB)进行定性与定量化分析。
3) cloning and sequencing
克隆测序
1.
Point mutations of p53 gene exon 7 were detected by cloning and sequencing assay.
通过克隆测序的方法检测p53基因外显子7上的点突变。
2.
Direct cloning and sequencing of restricted fragments with cohesive-ends by means of TA cloning;
对限制性酶切片段的粘性末端先用T4DNA聚合酶处理,变为平末端,然后用TaqTMDNA聚合酶在其3′末端加上A腺苷,即可利用T/A克隆载体进行直接克隆测序。
5) clone sequencing
克隆测序法
1.
Samples were detected by direct sequencing and clone sequencing,and the results were compared.
目的:了解PCR产物直接测序法与克隆测序法在单核苷酸多态性(SNP)检测上的差别。
6) BAC clone sequencing
BAC克隆测序
补充资料：PC
聚碳酸酯(双酚A型)化学名称2，2-双(4-羟基苯基)丙烷聚碳酸酯，是由酯交换法和光气化法制得。平均分子量3.57×104。无定形透明颗粒．无味、天臭、无毒。相对密度1．20-1.43。没有明显熔点，220-230℃呈熔融状态。透光率高，吸水性低，耐冲击，韧性好．蠕变小，制品尺寸稳定。耐热性和耐寒性较好．玻璃化温度149-150℃．热变形温度135℃(1．82MPa)、分解温度＞310 ℃，脆性温度－l00 ℃，使用温度-100～120 ℃。耐稀酸、耐油、不耐碱。溶于二氯甲烷、二氯乙烷、氯仿、三氯乙烯、四氯乙烷、四氢呋喃、三甲酚、磷酸三甲酯等。疲劳强度较低，容易产生应力开裂。具有优良的高温电性能，具有耐燃自熄性。拉伸强度 ＞60MPa, 伸长率＞60％，弯曲强度＞95MPa。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条