2) reporter gene
报告基因
1.
Transfection and expression of retrovirus-mediated reporter genes in retinal pigment epithelial cells in vitro;
逆转录病毒载体携带报告基因在色素上皮细胞的转染与表达
2.
Establishment and its application of a reporter gene-based screening cell model of NF-κB signal transduction to combat Alzheimer s disease;
基于报告基因和NF-κB信号通路的抗阿尔茨海默病药物细胞筛选模型的建立与应用
3.
Monitoring early toxicity of heavy metals including Hg using a HSE-SEAP reporter gene;
报告基因方法监测重金属汞及其化合物的早期毒性
3) report gene
报告基因
1.
Inducement of ginsenoside Rg_1 on expression of glucocorticoid receptor report gene in HL-7702 cells;
人参皂苷Rg_1诱导人肝HL-7702细胞糖皮质激素受体报告基因表达的作用
2.
Recombinant adeno-associated virus vector-delivered report gene expression in corneal endothelium;
重组腺相关病毒介导的报告基因在角膜内皮细胞中的表达
3.
Construction and identification of human Puma promotor luciferase report gene vector
人Puma启动子荧光素酶报告基因的构建和鉴定
4) reporter genes
报告基因
1.
Objective To construct bait vector pGBKT7-COPS3 in yeast two-hybrid system GAL4,and explore whether expression product of COPS3 has toxicity to host yeast cells and activates the reporter genes in the yeast two-hybrid system.
目的用COPS3基因片段构建酵母双杂交GAL4系统的诱饵载体pGBKT7-COPS3,并检测其表达产物对酵母细胞有无毒性及对报告基因有无激活作用。
2.
AIM: To construct a bait vector with Bit1 gene in yeast two-hybrid system GAL4, and assay whether Bit1 gene expression product affects the growth of host yeast cells and activates the reporter genes in the yeast two-hybrid system.
目的:用Bit1基因片段构建酵母双杂交诱饵载体,并检测其表达产物对酵母细胞有无毒性作用及对报告基因有无激活作用。
5) luciferase reporter gene
荧光素酶报告基因
1.
These DNA fragments were inserted into the pGL3-Basic which was the vector of luciferase reporter gene.
方法提取人内皮细胞基因组DNA,设计引物克隆EOLA1基因上游不同长度的基因片段,插入到含荧光素酶报告基因的载体pGL3-Basic中,经限制性内切酶酶切鉴定、测序。
2.
[Method] Clonging the promoters of CYP3A4 and CYP2B6 which contained the elements that hPXR, a kind of nuclear receptor, can recognize and bind, and inserting the trans-element to the upstream of firefly luciferase reporter gene of the pGL4.
方法利用双荧光素酶报告基因系统,将包含hPXR蛋白识别和结合调控元件的CYP3A4和2B6启动子序列插入报告基因上游,将表达载体和报告载体共转染HepG2细胞,用含有利福平或DMSO培养基培养48h后裂解进行双荧光素酶活性检测。
3.
Amplified apolipoprotein A-I(apoA-I) promoter gene(376 bp) containing essential transcription regulatory elements from human genomic DNA was fused upstream to the luciferase reporter gene and neomycin resistance gene in the constructed pGL3B-neo vector.
PCR扩增含有apoA-I基因的转录调控序列的启动子片断(376 bp),克隆入含有荧光素酶报告基因和新霉素抗性基因的pGL3B-neo载体中,构成受apoA-I启动子调控的重组报告基因质粒pGL3B-neo::apo,稳定转染人肝癌细胞株HepG2。
6) luciferase report gene
荧光素酶报告基因
1.
Methods The +495bp～+584bp of SCN5A promoter was amplified by polymerase chain reaction(PCR)and recombinated into pGL3-promoter luciferase report gene vector and transient transfected HEK293 cell and H9C2 cell.
方法应用聚合酶链反应扩增大鼠心肌SCN5A基因启动区+495bp~+584bp片段,与荧光素酶报告基因载体pGL3-promoter重组,以pGL3-promoter空载体为对照,瞬时转染人胚肾母细胞(HEK293)和小鼠胚胎心肌细胞(H9C2),检测荧光素酶活性。
2.
coli chromosome,the luciferase report gene was knocked in lacZ site of chromosome lac operon by using Red recombination system and selection-counterselection kan/sacB technology.
为了应用Red重组工程技术实现外源基因在大肠杆菌染色体上的表述,寻找染色体上外源蛋白的稳定高效表达位点,使用Red重组工程系统和kan/sacB无痕迹修饰技术,将易于定量分析的荧光素酶报告基因替换DY330染色体lac操纵子中的lacZ基因。
补充资料：报告基因
分子式：
CAS号：
性质：在研究启动子结构与功能时常使用一些有明显标记的结构基因作指示，如半乳糖苷酶基因、青霉素酰胺酶基因、荧光素酶基因、生长激素基因等。
CAS号：
性质：在研究启动子结构与功能时常使用一些有明显标记的结构基因作指示，如半乳糖苷酶基因、青霉素酰胺酶基因、荧光素酶基因、生长激素基因等。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条