Discussion:"The qi dynamic" is a fundamental question of traditonal chinese medicine,hot and cold stimulation to quantify the transmission of cold and heat will help drive changes of "the qi dynamic",which provides a theoretical basis,thereby drawing a line test to establish the methods to evaluate tradional Chinese medicine "qi".

Cortical responses of intra-oral cold stimulation measured with fMRI;

Conclusion: Cold stimulation can lead the disfunction of the nailfold microcirculation and decrease the microblood flow of nailfold.

Observation by scanning electron microscope on the structural changes of cartilage in chicken knee joint after cold stimulation;