1) freeze-thawing with liquid nitrogen
液氮快速冻融法
1.
Methods Human hepatocellular cancer cell line HepG2 were treated with different concentrations of leech extracts which were extracted by method of freeze-thawing with liquid nitrogen and contrasted with that by method of water extracting and ethanol precipitating.
方法以液氮快速冻融法制备水蛭提取物,以常规水提醇沉法水蛭提取物作对照,不同浓度体外干预人肝癌HepG2细胞,MTT法检测两种水蛭提取物对HepG2细胞增殖的抑制作用,并进行细胞计数,吖啶橙荧光染色法检测细胞凋亡情况。
2) rapid frozen thaw method
快速冻融法
1.
In order to obtain total DNA from Magnaporthe grisea for the study of molecular biology in a rapid, high efficiency and economic way, the authors have developed a new method——rapid frozen thaw method.
为了快速、简便和经济地获得稻瘟病菌的总DNA ,以用于分子生物学研究 ,我们建立了一种制备其总DAN的新方法———快速冻融法 。
3) Frozen-Thawing method
液氮冻融法
4) liquid nitrogen quick- freezing
液氮快速冻结
5) rapid freeze-thaw test
快速冻融
6) thawing and freezing test of liquid nitrogen
液氮冻融
1.
The measurement of amylose in glossy ganoderma sporule powder treated with ultrasonic and thawing and freezing test of liquid nitrogen was analysed by the method of phenol-sulfuric acid.
利用苯酚-硫酸法对超声波-液氮冻融处理的灵芝孢子粉多糖含量进行了测试,结果表明采用液氮进行淬冷破壁具有效果好,操作简便等特点;增加冻结-解冻循环次数在一定程度上能提高多糖含量,循环次数以4次左右为宜;将超声波处理法和液氮处理法很好地结合起来,其内容物释放更高,效果更佳,此方法更具实用价值。
补充资料：液氮
分子式：N2
分子量：28.01
CAS号：7727-37-9
性质：熔点-210°C。沸点-196°C。
分子量：28.01
CAS号：7727-37-9
性质：熔点-210°C。沸点-196°C。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条