Knockout of the Crystalline Inclusion Protein Gene cipA and cipB in Photorhabdus luminescens by RED Homologous Recombination

lincolnensis JLUa2,was generated by gene knockout of lmbU with homologous recombination.

coli W3110 chromosome using the plasmid as follow: Firstly, we knockout the lacI gene using Red-mediated recombineering with overlapping single stranded DNA oligonucleotides.

Construction and application of a vector for gene deletion in gram-negative bacteria

After the construction,the vector was used to carry out gene deletion experiment in the yeeZ gene from Citrobacter freundii.

Transformation of Escherichia coli and Bacillus thuringiensis and their plasmid curing by electroporation;

Plasmid curing experiment was carried out by using the sacB positive selection method and plasmid cured derivatives,2020D29,2020D8 and 7653RD were obtained.

The plasmid elimination methods that were applied in probing plasmid function of Mesorhizobium huakuii were introduced in this paper.

High temperature and the elimination agent were combined by plasmid elimination for a cadmium-resistant Lactococcus lactis strains,the amount and the time by using temperature and eliminaton agent acridine orange (AO) were approached on the effect of cadmium-resistant Lactococcus lactis plasmid.

Ever since the conception of plasmid elimination was mentioned,more and more researches have been carried out and acquired some advancement.