1) shuttle vector pMG36e
穿梭载体pMG36e
1.
lactis shuttle vector pMG36e,resulting in the plasmid pMG36e-NisI.
【方法】根据GenBank报道的乳链菌肽Nisin抗性基因nisI的序列,以pLEB590为模板,设计特异性引物,PCR扩增出nisI片段,经TA克隆、酶切鉴定及测序之后,亚克隆至大肠杆菌-乳酸乳球菌穿梭载体pMG36e中,重组子命名为pMG36e-NisI,再将pMG36e-NisI电转化至对Nisin敏感的乳酸乳球菌MG1363中。
2) Shuttle vector
穿梭载体
1.
Construction of shuttle vector consisted of human autophagic gene hAPG12 and its expression in yeast;
含人自噬基因hAPG12穿梭载体构建及其在酵母自噬中的作用
2.
Construction of Deinococcal bacteria-Escherchia coli shuttle vector and expression of Luciferase gene
抗辐射菌属-大肠杆菌间的穿梭载体的构建及荧光基因的表达
3.
Then, the fragment containing PpsbA and rhTNF cDNA was linked with the shuttle vector PDC_8, and resulted in expressing vector PDC_TNF.
应用ＤＮＡ重组技术，将人肿瘤坏死因子（ｒｈＴＮＦ）ｃＤＮＡ插到质粒ＰＲＬ４３９的ＰｐｓｂＡ启动子下游，得到中间载体ＰＲＬ＿ＴＮＦ；再把ＰＲＬ＿ＴＮＦ上含ＰｐｓｂＡ和（ｒｈＴＮＦ）ｃＤＮＡ的片段连到穿梭载体ＰＤＣ＿８上，构建成穿梭表达载体ＰＤＣ＿ＴＮＦ。
4) shuttle vector pNW33N
pNW33N穿梭载体
5) shuttle expression vector
穿梭表达载体
1.
Construction of shuttle expression vector in eukaryocyte plant cells;
Kringle5的真核植物细胞穿梭表达载体的构建
2.
Development of a Shuttle Expression Vector, pFC9, for Cryphonectria Parasitica;
一个可在板栗疫病菌中自主复制的穿梭表达载体
3.
GFP labeling shuttle expression vector pW425et-GFP was constructed by inserting green fluorescent protein(GFP)based on erythromycin drug resistance gene /thyA gene dipl-bolting pressure shuttle expression vector pW425et.
以红霉素耐药性基因/thyA基因双筛选压力大肠杆菌—乳酸杆菌穿梭表达载体pW425et为基本骨架,将绿色荧光蛋白(GFP)基因插入该载体,构建GFP标记的穿梭表达载体pW425et-GFP。
6) Bi-shuttle vector system
双穿梭载体系统
补充资料：穿梭
织布机上梭子往来穿越。比喻快速或往来频繁：船驶起来就像织布穿梭、缝衣透针一般快。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条