Objective To study the relativity of multidrug resistance gene 1(MDR1) gene expression and acquired tolerance to arsenic in human hepatocyte cell line(L-02).

Methods Human hepatocyte cell line(L-02) were exposed to low-level arsenite for 6 weeks,3-[4,5-dimethy Ithiazol-2-yl]-2,5-diphenyl tetrzolium(MTT) was used to detect the survival rate and LC50 of arsenic-exposed cells and the control which can reflect the change of arsenic tolerance.

Antagonistic effect of scutellarin on toxicity of selenium in human liver cells L-02;

Object: investigate the formation of lipid droplets and expression ofcholesterol rate-limiting enzyme—HMG-CoA reductase and transported protein— caveolin-l,detect the amount of cholesterol on human L-02 hepatocyte under the control of curcumin, to find out the effects of curcumin on cholesterol synthesis and transport.

Objective To investigate the possible mechanisms of curcumin increasing cholesterol transport in the fatty changed human L-02 hepatocyte.

AIM: To detect the inhibition effect of nitidine chloride on proliferation of human liver cell L-02 and human kidney cell 293 and the protective effect of acidic fibroblast growth factor(aFGF) on human liver cell L-02 and human kidney cell 293 damaged by nitidine chloride in vitro.

Establishment of experimental model for DNA damage tolerance induced by HQ in L-02 hepatic cells;

Effect of hydroquinone on the expression of ubiquitin-conjugating enzyme Rad6B in human L-02 hepatic cells

Methods MTT assay was used to detect the effects of HQ with various concentrations(0,5,10,20,40,80,160 and 320 μmol/L)on the survival rate of L-02 hepatic cells for 24 h.