In order to improve the convergent velocity of CSA,the operators of clone expansion and immune gene operation were introduced into CSA.

Clone copy operator,immune gene operator and clone selection operator were performed during the evolutionary.

Preparation of monoclonal antibodies against the MPB64 antigen of Mycobacterium bovis by gene immunization;

Experimental study on antitumor effects of GM-CSF gene immunization on mouse myeloma;

Influence of somatostatin gene immunization on growth and GH and IGF-Ⅰ of Hu lamb;

Genetic immunization of BALB/c mice with extracellular domains of human TSH receptor provides a model of Graves disease;

Generation of mouse anti-human organic anion transporter 3 polyclonal antibody by improved genetic immunization;

Preparation of the anti-human chorionic gonadotrophin β-subunit monoclonal antibodies based on the genetic immunization with recombinant plasmid and characterization of its biological properties;

DNA immunization of mice with plasmid encoding mimic epitopes of hypervariable region 1 of hepatitis C virus;

DNA immunization has recently been proven to induce both humoral and cellular immune responses against a range of viruses, so it shows a promising approach to prevention and cure of persistent viral infection.

These studies on aspects of gene immunity experiment of infection by virus, tumour and parasite, the mechanism of gene immunity and its safety, are widely developed abroad.

The gene immunity may prepare the monoclonal antibody to aim directly at the natural epitope and save 2-3 months.