Construction and in vitro expression of bicistronic DNA vaccines containing hepatitis B virus core antigen and Flt3 ligand genes;

Objective To activate peripheral blood B lymphocytes by CpG-oligodeoxynucleotides(CpG-ODN) and load HBcAg 18-27 peptide to make them as antigen presenting cells(APC),then induce hepatitis B virus(HBV)-specific cytolytic T lymphocytes(CTL) from peripheral blood monocular cells(PBMC) of healthy human.

Preparation and application of discoloration dilution detecting anti-HBc antibody;

2%) belonged to three typical HBV infected models:modelⅠwas HBsAg (+),HBeAg (+) and HBcAb (+);model Ⅱ was HBsAg (+),anti-HBe (+) and anti-HBc (+); model Ⅲ was HBsAg (+) and anti-HBc (+).