Methods The immunohistochemiscal S-P method techniques were amplified to study the expression of aromatasecy P450,estrogen receptor(ER)and progesterone receptor(PR)in the tissues of endometrial from the observed group of 116 cases,including 30 cases of simple endometrial hyperplasia,32 cases of complex endometrial hyperplasia and 54 cases of endometrial atyp.

Sixteen healthy,non-pregnant mature female SD rats of 220 to 250g in body weight were divided randomly into 4 groups of 4 each by vaginal smear,and the expression of neuron-specific enolase(NSE) in uterus of the rats during estrus cycle were studied by immunohistochemical SP method.

Accordingly, The expression and changes of ghrelin-imunopositive (ghrelin-ip) cell and IFN-γwere detected with immunohistochemical SP method in healthy ostrich’s small intestine during 3 days, 3 weeks and 11 months.

To study the localization of estrogen receptor α(ERα) and its action in the process of reproduction during the different period of reproduction in goat ovary,supersensitive immunohistochemistry SP method was used to detect the distribution of the ERα immunoreactivity(ERα-ir) during the different period of reproduction in the goats ovary.