Isolation and Identification of necrotic enteritis

Isolation and genotypic identification of Clostridium perfringens from environments of animal houses;

Identification of toxin types of Clostridium perfringens isolated from domesticated pig, sheep and cattle by multiplex PCR;

SDS PAGE analysis of cell proteins and membrane proteins of Clostridium perfringens;

Development of Propolis-adjuvant Inactivated Vaccine against Clostridium welchii of Rabbits;

Preparation and Application of α- Toxin Gene Probe of Clostridium welchii;

Cloning and expression of α-toxin gene of Clostridium welchii isolated from Guizhou;

Diagnosis And Investigation on Sheep CL.septicum And CL.welchii Mixed Infection;

To diagnose the infected disease of grazing sheep,some tests were selected as follows,investigation of clinical pathology,clinical examination,pathologic anatomy,smear examination,separated culture,animal inoculation,and serum shapng,which showed that the sheep was suffered from toxaemia of intestine infected with CL.

Multiplex PCR for detection of rabbit EPEC and Clostridium Perfringen;

A multiplex polymerase chain reaction was optimized to simultaneously detect two toxinal gene of REPEC and clostridium perfringen in this research.

A multiplex polymerase chain reaction was optimized to simultaneously detect two toxin gene of REPEC and clostridium perfringen in this research.

Studies and Applications on the Two Combined Vaccine of Rabbit Viral Hemorrhagic Disease and Clostriclium Welchii Type A;