Based on the sequence information of the ω3-fatty acid desaturase genes from Arabidopsis thaliana, Nicotiana tabacum, Betula pendula and Ricinus communis, a cDNA putatively encoding a plastidial ω3-fatty acid desaturase (JcFAD7) was isolated from Jatroha curcas with reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) method.

To study the function of peanut △12-fatty acid desaturase,the full-lengh cDNA of AhFAD was amplified by RACE from peanut seed.

In order to test the possibility of reconstructing the Pichia pastoris strain GS115 into a PUFAs-producing strain by genetic engineering,Δ~(12)-fatty acid desaturase gene from Rhizopus arrhizus was subcloned into the intracellar expression vector pPIC3.

To study the function of peanut Δ12 fatty acid desaturase(Δ12 FAD),the cDNA of AhFAD2B was amplified by RT-PCR from immature peanut seeds,then cloned into the shuttle expression vector p416 to generate the recombinant vector p4AFAD2B.

Cloning and expression of a △6-fatty acid desaturase gene from Rhizopus stolonifer in Saccharomyces cervisiae;