2) endothelial cell-conditioned medium
内皮细胞条件培养液
1.
Mononuclear cells(MNCs)from human umbilical cord blood were cultured by using culture system supplemented with endothelial cell-conditioned medium.
为了建立从人脐血分离培养两种内皮祖细胞的方法和培养体系,从脐血分离单个核细胞,在含有内皮细胞条件培养液的体系中培养得到两种细胞,利用摄取乙酰化低密度脂蛋白(acetylated low-density lipoprotein,DiI-Ac-LDL)和结合欧洲荆豆凝集素(Ulex European Agglutinin1,UEA-1)进行鉴定,并进一步用免疫细胞化学、RT-PCR、流式细胞术对它们进行内皮细胞相关的表型检测。
3) cerebral microvascular endothelial cell
脑微血管内皮细胞
1.
Effect of effective components of Huanglian Jiedu decocting on NF-κB in cultured rat cerebral microvascular endothelial cells injury induced by hypoxia and reoxygenation;
黄连解毒汤有效成分对缺氧/复氧时脑微血管内皮细胞核因子-κB的影响
2.
Structure-activity relationship of phenylallyl compounds inhibiting PGE_2 release in mouse cerebral microvascular endothelial cells induced by IL-1β;
桂枝汤有效成分苯丙烯类化合物干预IL-1β刺激小鼠脑微血管内皮细胞释放PGE_2的构效关系
3.
Protective action of effective components of Huanglian Jiedu decoction on hypoxia and reoxygenation injury in cultured rat cerebral microvascular endothelial cells;
黄连解毒汤有效成分对缺氧/复氧时脑微血管内皮细胞的保护作用
4) brain microvascular endothelial cells
脑微血管内皮细胞
1.
Protective effect of hirudin against apoptosis on cultured rat brain microvascular endothelial cells;
水蛭素对凝血酶所致脑微血管内皮细胞损伤的保护作用
2.
Effect of brain microvascular endothelial cells on differentiation of co-cultured mesenchymal stem cells under hypoxic conditions;
缺氧条件下大鼠脑微血管内皮细胞对共培养的人间充质干细胞分化的影响
3.
Aim To investigate the effects of curcumin on the adherence between isolated rat brain microvascular endothelial cells(BMECs)and leukocytes induced by TNF-α in vitro.
目的研究姜黄素(curcumin)对脑微血管内皮细胞(brain microvascular endothelium cell,BMEC)和白细胞粘附的影响。
5) Brain microvascular endothelial cell
脑微血管内皮细胞
1.
Effects of Qingkailing effective components on nuclear factor-kappa B in an ischemia-reperfusion injury model of rat brain microvascular endothelial cells in vitro
清开灵有效组分对大鼠脑微血管内皮细胞体外缺血再灌注损伤模型核转录因子-κB的影响
2.
Objective:To observe the expression of matrix metalloproteinase-9 mRNA of cultured rat brain microvascular endothelial cells(MVEC),expecting to explore the mechanisms of QingKaiLing effective components protecting brain from injury of inflammatory cascade in cerebral ischemia diseases.
目的:观察大鼠脑微血管内皮细胞缺血再灌注损伤MMP-9表达的影响,探讨清开灵有效组分保护血脑屏障阻抑脑缺血再灌注损伤的作用途径。
3.
ObjectiveTo study the influence of conditioned medium of rat brain microvascular endothelial cells on mitochondrial function of cortical neurons and the protective effect of Tongluo Jiunao Injection(TJI)on it.
目的探讨脑微血管内皮细胞条件培养液对损伤神经元线粒体功能的影响,以及通络救脑注射液对此过程的干预作用。
6) human brain microvascular endothelial cell
人脑微血管内皮细胞
1.
Methods Human recommbinant MIP-1α(rhMIP-1α) was incubated with human brain microvascular endothelial cell (HBMEC) monolayer and the permeability of HBMEC monolayer was observed with immunofluorescence, TEER and HRP flux methods.
方法以重组人MIP-1α直接作用于人脑微血管内皮细胞(HBMEC),免疫荧光方法检测紧密连接蛋白ZO-1的分布变化、跨内皮细胞电阻、HRP穿过HBMEC单层的改变、HBMEC细胞CC趋化因子受体5(CCR5)的表达,以及MIP-1α中和抗体和分泌MIP-1α的模式细胞(6T-CEM)与HBMEC单层共同温育时ZO-1的分布变化。
2.
We have showed the stronger ability to migrate through human brain microvascular endothelial cells (HBMECs) and a significantly higher macrophage inflammatory protein-1α (MIP-1α) expression in peripheral T lymphocytes of AD patients than age-matched healthy subjects.
我们研究曾发现,AD病人外周血T淋巴细胞穿过人脑微血管内皮细胞(HBMEC)单层能力高于同龄正常人,其T淋巴细胞巨噬细胞炎症蛋白1α(MIP-1α)表达明显增高。
3.
To reveal the expression of vascular endothelial growth factor (VEGF) under conditions that cause endoplasmic reticulum (ER) stress, human brain microvascular endothelial cells (HBMEC) were treated with tunicamycin at different concentration in vitro.
为观察内质网应激条件下血管内皮细胞生长因子的表达情况,用不同浓度的衣霉素处理体外培养的人脑微血管内皮细胞,建立内质网应激模型,采用RT-PCR、蛋白质免疫印迹以及免疫细胞化学的方法检测了细胞内血管内皮细胞生长因子的表达。
补充资料：Earle培养液或营养液
Earle培养液或营养液
该液即EBSS，Earle’s平衡盐溶液(balanced salt solution)，含氯化钙(CaCl2)(无水)1.8mM，氯化钾(KCl)5.3mM，硫酸镁(MgSO4)(无水)0.8mM，氯化钠(NaCl)117.0mM，碳酸氢钠(NaHCO3)26.0mM，磷酸钠(NaH2PO4·H2O)1.0mM，D-葡萄糖(Dglucase)5.6mM，酚红(phenol red)0.03mM，液体1000ml。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条