1) specific PCR
特异性PCR
1.
Therefore,primers specific for the U3 sequences of exJSRV were designed for the specific PCR and nested PCR(n-PCR).
健康羊基因组中存在15~20拷贝的内源性前病毒enJSRV,内外源性前病毒的结构基因高度相似,而在U3区存在差别,因而,设计了针对exJSRVU3区的特异性引物并在国内首次建立了一步法特异性PCR检测法及套式PCR检测法。
2) Allele-specific PCR(AS-PCR)
位点特异性PCR(AS-PCR)
4) group-specific PCR
群特异性PCR
5) PCR-seqence specific primers (PCR-SSP)
PCR-序列特异性引物(PCR-SSP)
6) Methylation specific PCR
甲基化特异性PCR
1.
Methods The methylation status of CpG island of AR gene promoter in 3 leukemic cell lines and 15 leukemic bone marrow cells was detected by methylation specific PCR(MSP).
方法应用甲基化特异性PCR技术(methylation specific PCR,MSP)检测3种白血病细胞株及15例患者骨髓标本雄激素受体基因甲基化状态。
2.
The CpG island methylation status of DAPK was examined by means of methylation specific PCR(MSP),and the products of MSP were subjected to TA cloning.
方法收集40对新鲜的膀胱癌组织及其癌周正常组织;采用甲基化特异性PCR(methylation specific PCR,MSP)检测DAPK基因启动子区CpG岛的甲基化状态;并对MSP产物进行TA克隆。
3.
Methylation status was evaluated by methylation specific PCR,T-A cloning and sequence analysis.
方法用5- 杂氮-2’-脱氧胞苷对Hep-2人喉癌细胞系处理后,甲基化特异性PCR(MSP),T-A克隆测序分析死亡相关蛋白激酶(death-associ- ated protein kinase,DAPK)基因甲基化状态,逆转录聚合酶链反应、免疫细胞化学、流式细胞仪检测用药前后DAPK mRNA和蛋白的表达情况及细胞凋亡和周期的变化。
补充资料:特异
1.特别怪异的现象。 2.特殊,不同一般。 3.特别优异。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
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