Methods Formalin-fixed and paraffin-embedded(FFPE)specimens from 75 NSCLC patients with stage Ⅲ B/IV were obtained and the total RNA was extracted by using Trizol one-step method.

Introduction FFPET(Formalin Fixed and Paraffin Embedded Tissues) are very important individual archives which are commonly used for disease diagnose and scientific research in clinic pathology, forensic pathology and other subjects, so it would be a reliable key evidence for cases of insurance fraud or inheritance of property or medical disputes, and other civil or criminal cases.

BackgroundFFPET(Formalin Fixed and Paraffin Embedded Tissues)are very important individual archives which are commonly used for disease diagnose and scientific research in clinic pathology,forensic pathology and other subjects,so it would be a reliable key evidence for cases of insurance fraud or inheritance of property or medical disputes,and other civil or criminal cases.

Objective To investigate Mg 2+ ionic concentration influence PCR that the DNA extraction from paraffin embedded tissues.

Detection of classical swine fever virus RNA by nested RT-PCR from paraffin-embedded tissues;

An improved method to recover high quality genomic DNA from paraffin-embedded tissues

Purpose To investigate the application value of universal linkage system (ULS) in compa- rative genomic hybridization (CGH) of formalin-fixed, paraffin-embedded tissue.

Comparison of two DNA extraction methods for paraffin embedded tissues

Owing to the DNA degradation induced by formalin and the obstruction of paraffin to DNA extraction, it is difficult to recover high-quality DNA from Formalin fixed and paraffin embedded tissue(FFPET).

Puropose To establish the tissue microarray (TMA) techniques of frozen and paraffin embedded tissues of lung carcinoma and to explore their application in gene expression analyses.