Objective: To develop further applications of magnetic polymer microspheres in biomedical field and to prepare the Fe_3O_4/PANI anti-oxidation water-based nano-magnetic fluid.

Nanosize Fe3O4 particles were prepared by the chemical co-precipitation method,without nitrogen protection;and the Fe3O4 nano-particles were coated by PANI through in-situ synthesis,then the Fe3O4/PANI material was prepared,which could also be called Fe3O4/PANI anti-oxidation water-based nano-magnetic fluid.

Construction of a recombinant eukaryotic expression vector pEGFP-AFP-TK and transfection into hepatoma cells by PEG-PEI/Fe_3O_4 magnetic nanoparticles;

Object To prepare PEG-PEI Fe_3O_4 nano-magnetic fluid and invest its characteristics.

Development in preparation of water-based magnetic liquid with chemical coprecipitation method;