Construction and immunity response of a suicidal DNA vaccine with E gene of Japanese encephalitis virus in mice

Suicidal DNA vaccine has become the focus of DNA vaccine researches not only advantages of conventional DNA vaccine,but also because of its advantages of biosafety and enhanced immunogenicity.

45 kb DNA fragment containing the 26S promoter of Semliki Forest virus(SFV) and a linker was amplified by PCR and inserted into the multiple clone sites of pSCA1,a suicidal DNA vaccine vector based on SFV replicon,resulting in a modified vector pSCA2 to drive the co-expression of double genes.

Synthesis Poly(LacNAc/Gln - co - Gln) as DNA Vaccine Adjuvant;

The prime-boost strategy with mucosal DNA vaccine and killed oil-emulsified vaccine against H9 subtype of avian influenza virus;

Immunotherapeutic effect of Ag85A DNA vaccine and Ag85B DNA vaccine on bladder tumor in rats;

Immune mechanism of DNA vaccines and progress on Newcastle disease virus (NDV);

Research advances in genetic adjuvant for DNA vaccines and its application in fish vaccines;

Study of immune responses induced by human papillomavirus type 18 L1-E6 and L1-E7 chimeric gene DNA vaccines in mice;