Expression and Immunological Activity of E2 Epitope of Classical swine fever virus;

Pathogenicity of classical swine fever virus GX-3/98 strain and immunization relativity with attenuated hogcholera virus Chinese C strain;

Kinetics of peripheral blood leukocytes apoptosis and changes of CD4~+ and CD8~+ T lymphocyte subpopulations of pigs infected with classical swine fever virus;

The recombinant retroviral vector pBABE-puro-E2 was constructed by inserting classical swine fever virus(CSFV) Shimen strain E2 gene into pBABE-puro.

A classical swine fever virus(CSFV) E2 gene was codon-optimized according to the Sf9 cell codon usage.

A truncated gene encoding the major antigenic domains of E2 protein of classical swine fever virus(CSFV) was amplified by RT-PCR from the genomic RNA of CSFV C-strain and cloned into pPROEX-HTb expression vector to obtain recombinant pPROEX-tE2.