1) suppression subtractive hybridization(SSH) cDNA library
抑制性消减杂交(SSH) cDNA文库
2) suppression subtractive hybridization cDNA library
抑制差减杂交cDNA文库
3) cDNA selected-subtractive suppression library
cDNA差减抑制杂交文库
1.
In this study,using Shixin828,the wheat cultivar with high phosphorus utilization efficiency identified previously to be the experimental material,a root cDNA selected-subtractive suppression library enriched the response genes of deficient-Pi stress at various time-points was constructed.
以前期工作中鉴定的磷高效小麦品种石新828为材料,构建了富集不同低磷处理时间点特异表达基因的根系cDNA差减抑制杂交文库。
4) Subtractive cDNA library
消减cDNA文库
1.
In order to obtain an efficient subtractive cDNA library with larger fragments of age related cataract, an improved subtractive hybridization with biotin labeling and magnetic bead isolation to obtain the subtractive cDNA was used.
为构建含较多大片段的高质量的老年性白内障消减cDNA文库 ,利用生物素标记、磁珠分离的改良消减杂交法获得差异cDNA 。
2.
In order to obtain a quality subtractive cDNA library of age related cataract which contains more large fragments from small amounts of sample, an improved subtractive hybridization which using magnetic beads isolation and biotin labelling was used to obtain the subtractive cDNA.
为从少量标本中获得含较多大片段的、高质量的老年性白内障消减cDNA文库 ,利用磁珠分离、生物素标记的改良消减杂交法获得差异cDNA ,利用选择性PCR法扩增其中大片段差异cDNA ,从而成功构建老年性白内障消减cDNA文库 。
3.
The skin follicle subtractive cDNA library of the Inner Mongolian cashmere goat was constructed by suppression subtractive hybridization.
应用抑制性消减杂交技术成功构建了内蒙古绒山羊胚胎期和兴盛期皮肤毛囊的消减cDNA文库。
5) cDNA subtractive library
cDNA消减文库
1.
The cDNA subtractive library of the mantle of abalone vitamin E deficiency-treated was constructed using the method of suppression subtractive hybridization(SSH).
04)mm,240 d后,采用抑制消减杂交法(Suppression Subtractive Hybridization,SSH)构建了维生素E缺乏组与正常组外套膜组织差异表达的cDNA消减文库。
2.
20 differential ests(expressed sequence tags) which are associated with the production of citrinin were screened from a cDNA subtractive library of Monascus through bioinformatics software,then BLAST software was applied to perform the homologous alignment and functional analysis of them.
通过分子生物学软件,从产桔霉素相关的红曲菌cDNA消减文库中找出20个与桔霉素合成相关的差异序列,并用BLAST软件对这些序列进行同源比对,分析其功能。
6) SUBTRACTIVE CDNA LIBRARY
差减cDNA文库
1.
Screening of differentially expressed genes in heat-stressed mouse testis by construction of a subtractive cDNA library
用差减cDNA文库筛选由热激导致的小鼠睾丸中差异表达的基因
2.
In order to obtain Peyer s patch specially expressed genes,we constructed a subtractive cDNA library using suppression subtractive hybridization from Peyer s patch(PP) and non-PP tissue.
采用抑制性差减杂交(suppression subtractive hybridization,SSH)方法,构建山羊羔肠道淋巴集结与其非PP肠壁组织的差减cDNA文库,以期找到在山羊羔肠道淋巴集结中特异表达的与免疫相关的基因。
补充资料：抑制杂交翻译
分子式：
CAS号：
性质：又称杂交扣留翻译。能使一克隆DNA与它所编码的蛋白质相关联的方法。这个方法基于下述事实：以RNA在与它的DNA互补体杂交后就不会在无细胞的翻译系统中指导蛋白质的合成。无细胞系统所合成的蛋白质，包括一个特定的杂交mRNA，就能与未发生同DNA杂交的系统所合成的蛋白质相比较，其差别就表示了由所研究的mRNA编码的蛋白质的性质。
CAS号：
性质：又称杂交扣留翻译。能使一克隆DNA与它所编码的蛋白质相关联的方法。这个方法基于下述事实：以RNA在与它的DNA互补体杂交后就不会在无细胞的翻译系统中指导蛋白质的合成。无细胞系统所合成的蛋白质，包括一个特定的杂交mRNA，就能与未发生同DNA杂交的系统所合成的蛋白质相比较，其差别就表示了由所研究的mRNA编码的蛋白质的性质。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条