1) coculture
共培养体
1.
In order to research into its physiological and ecological characteristics and to lay the scientific basis for the development of highly efficient anaerobic biological treatment technology,the syntrophic acetogen cocultures were enriched and Selective bred by the Selective medium which is mixed by the Propionic acid and butyric acid from the anaerobic activated sludge with methanogens.
基于以上认识和研究现状,本论文开展了产氢产乙酸互营共培养体的分离筛选工作,并以此为基础,对所筛选的共培养体进行了生理生态特性研究,并进一步探索了以其强化厌氧生物处理系统效能的可行性。
2.
In order to study HPA\'s physiological and ecological characteristics and to establish the scientific basis for the development of highly efficient anaerobic biological treatment processes, the syntrophic acetogen cocultures are enriched and selectively bred from the anaerobic activated sludge with methanogens by selective medium, using propionic and butyric acids as the only carbon source.
为了深入了解其生理生态特性,为开发高效的厌氧生物处理技术提供理论基础,采用丙酸和丁酸混合培养基,以具有甲烷发酵功能的厌氧活性污泥为出发菌群,进行了产氢产乙酸菌互营共培养体的选育,并借助于PCR-DGGE技术对其菌群结构进行了解析。
2) co-culture
共培养体系
1.
Construction of CTECs Co-culture System and Study of Cytopathogenic Effects on CTECs by IBV;
鸡气管黏膜上皮细胞共培养体系的建立及IBV致细胞病变作用的研究
2.
lemaneiformis,especially their fresh tissues,significantly interfered with the growth of the co-cultured microalgae.
研究了2种大型海藻石莼(Ulva pertusa)和江蓠(Gracilaria lemaneiformis)对2种赤潮微藻:东海原甲藻(Prorocentrumdonghaiense)和塔玛亚历山大藻(Alexandum tamarense)生长的影响以及2种微藻之间的相互作用,结果发现:①在大藻(石莼或江蓠)-微藻(东海原甲藻或塔玛亚历山大藻)的共培养体系中,石莼和江蓠均能明显影响与其共培养的微藻的生长,石莼对微藻生长的影响强于江蓠的作用。
3) co-culture system
共培养体系
1.
Methods The rat proximal tubular epithelial cells and osteoblasts were culture to establish the co-culture system in vitro.
方法体外分离培养大鼠近端肾小管上皮细胞与头盖骨成骨细胞;用甲状旁腺激素(PTH)、137Cs-γ射线及25(OH)D3预作用前者,再与后者分为4组共培养:①单培养组(成骨细胞单培养)、②共培养组(肾小管上皮细胞与成骨细胞共培养)、③刺激共培养组(肾小管上皮细胞经PTH+25(OH)D3处理,再与成骨细胞共培养)、④抑制共培养组(肾小管上皮细胞经137Cs-γ射线照射+25(OH)D3处理,再与成骨细胞共培养);并用RT-PCR方法检测共培养体系中成骨细胞的BGP、ALP、ColI、RANKL与OPG mRNA表达。
2.
lemaneiformis co-culture system when the initial cell density of G.
实验室条件下研究了共培养体系中龙须菜(Gracilaria lemaneiformis)对锥状斯氏藻(Scrippsiella trochoidea)生长的影响,并对其作用机制进行了初步探讨。
3.
Human bone marrow mesenchymal stem cells were induced towards pancreatic islet progenitor cells by co-culture system.
利用共培养体系诱导骨髓间充质干细胞向胰岛祖细胞的初步转化，探讨骨髓间充质干细胞在糖尿病治疗领域的价值。
4) in vitro co-culture
体外共培养
1.
A basic in vitro co-culture system for ovine male germ cells has been established in this study.
本研究初步建立了绵羊雄性生殖细胞体外共培养系统,同时为从分子水平对共培养系统中各级生殖细胞的存活进行检测,首次成功克隆了绵羊各级生殖细胞的特异标记基因,如E型钙粘连蛋白基因(E-cadherin gene,cdh1)、联会复合体蛋白3基因(synaptonemal complex protein-3 gene,scp3)、过渡蛋白1基因(Transition protein-1 gene,tnp1)和过渡蛋白2基因(Transition protein-2 gene,tnp2),并利用得到的cDNA序列进行引物设计,用于转录表达分析来鉴定绵羊生殖细胞共培养系统中的各级生殖细胞。
5) Embryo co-culture system
胚胎共培养体系
补充资料：共培养
共培养
按照一般体外授精、胚胎移植的常规，是将2～8分裂球阶段的胚胎转移到子宫腔内；而在自然妊娠过程，卵子和精子在输卵管壶腹部相遇，结合，受精卵在输卵管内分裂约3天后形成桑椹体才下降到子宫腔内。这也可能是体外受精、胚胎移植中成功率不高的原因。所谓共培养指采用人输卵管壶腹部上皮细胞，在HamF10或Earle培养液中培养6～7天形成结合—片的单层。在这样共培养条件下，78%胚胎可达到桑椹体阶段，69%可达到胚泡阶段。也可用输卵管上皮细胞飘流的培养液。另外，也有人应用成熟卵泡的颗粒细胞或子宫内膜细胞进行共培养，这些都在研究中。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条