2) bidirection expression vector
双向表达载体
1.
The cDNAs of SIV 8 gene segments were synthesized by RT-PCR and cloned into the RNA polymerase I/II bidirection expression vector PHW2000 independently, resulting in 8 recombinant plasmids.
将猪流感病毒8个基因节段经RT-PCR合成cDNA后,分别克隆到RNA聚合酶I/II双向表达载体PHW2000中,构建成8个重组质粒。
2.
The cDNAs of SIV 8 gene segments were synthesized by RT-PCR and cloned into the RNA polymerase Ⅰ/Ⅱ bidirection expression vector PHW2000 independently,resulting in 8 recombinant plasmids.
方法:将猪流感病毒8个基因节段经 RT-PCR 合成 cDNA 后,分别克隆到 RNA 聚合酶Ⅰ/Ⅱ双向表达载体 PHW2000中,构建成8 个重组质粒。
3) retroviral vector
逆转录病毒表达载体
1.
The retroviral vector carrying both bax and EGFP gene was constructed using PCR and enzyme digestion etc, then this new vector pLBaxSN-IRES2-EGFP was used to transfect Hela cell to establish a new Hela strain, and the expression of the exogenous bax gene was verified by fluorescence microscope and Weste.
[目的]构建带有报告基因EGFP的bax基因逆转录病毒表达载体,建立高表达bax基因的Hela细胞。
2.
Objective To clone AC133-2, construct retroviral vector.
目的克隆人AC133-2全长基因,构建PGEZ-Term-AC133—2逆转录病毒表达载体。
4) bicistronic vector
双表达载体
1.
Enhancement of immune response to HCV core gene by GM-CSF gene with bicistronic vector;
双表达载体研究GM-CSF对HCVC基因免疫应答的影响
2.
Two genes were cloned to upstream and downstream internal ribosome entry site(IRES) sequences of pIRES, a bicistronic vector, to construct bicistronic plasmids.
方法:将HBcAg和FL胞外段的基因用PCR方法扩增并分别引入相应的限制性内切酶酶切位点,然后克隆入真核双表达载体pIRES,获得含双基因片段的表达质粒;再取该表达质粒中的内部核糖体切入位点(IRES)序列和基因片段克隆入pJW4303载体中,获得相应的双表达核酸疫苗,经筛选鉴定后,以293T细胞瞬时表达检测两基因的表达水平。
6) binary expression vector
双元表达载体
1.
Cloning of promoters for nitrate-induced genes from rice and construction of plant binary expression vectors;
水稻中受硝酸盐诱导的基因启动子的克隆与植物双元表达载体的构建
2.
Cloning of foot-and-mouth disease virus structural gene vp1 and construction of binary expression vector in plants;
口蹄疫病毒vp1基因的克隆与植物双元表达载体的构建
3.
The binary expression vector of PsENOD12A recombined with the pea lectin gene PsLectin was successfully constructed.
将PsENOD12A基因和豌豆凝集素基因psl重组进了双元表达载体。
补充资料：高低分分段双向截除法
高低分分段双向截除法
即在考核打分时为消除考核者主观打人情分的影响，而对考核得出的结果按所有考核成员得出分值的平均值的一定比例限定高低分区间，对超过此区间的考核分数的使用进行限定，在统计成绩时而不予考虑、计算。此种方法可控制打分者在一定程度上约束自己的打分行为，将其控制在一定程度内，否则其打出的分数将视为无效，实际上也即相当于放弃了自己的投票权。由于实际考核工作中，评委主观打分和打人情分的倾向较普遍，因而对于优化考核有一定意义。但此种方法对考核者人数较多的情况下更适用，而考核者人数较少时（更易受事前操纵、考核者信息泄露风险系数增加、恶意评分者博弈动机增强等因素影响），其有效性将会降低。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条