To build a bioluminescent method for the determination of microbial ATP in the tissues ofcorpse.

Objective To build a bioluminescent method for the determination of microbial ATP in the tissues of corpse and study the relationship between changes of microbial ATP in livers of rats and the postmortem interval(PMI).

ATP bioluminescence technique has been widely used as a rapid method for detection of bacteria in sample,however,such a phenomenon that ATP bioluminescence value of irradiated sample was higher than that of control was observed.

Moreover,the combination of ATP bioluminescence and IMS was correlated well with conventional culture methods,R2=0.

4),Tris-HCl,PBS,KH 2PO 4-NaOH and Na 2HPO 4-C 6H 8O 7,and five different chemical substances with the same mass concentration(10 g·L),NaCl,KCl,NaOH,MgCl 2 and NaH 2PO 4 on ATP bioluminescence were compared.

Research on rapid detecting microbial count in raw milk with ATP bioluminescence;

ATP bioluminescence is one kind of rapid detection method for microorganisms, since it is easy, highly sensitive and timely, etc.

Objective To apply the ATP bioluminescence assay for evaluating the cleaning degree of medical instrument.

Objective To compare the sensitivity of ATP bioluminescence assay with Endocheck test and occult blood test(OB)for detection of blood residue on medical instrument .

Objective: To study the inhibition of proliferation of LAAO purified from King Cobra venom on the cultured tumor cells which were tested by traditional assays—the inhibition of proliferation on the cultured tumor cells in vitro and ATP-Bioluminescence assay, and to explore its possible antitumor mechanism by induced- apoptosis on tumor cells .

ATP-Bioluminescence method allowed the rapid quantification of microorganisms by measuring their intracellular ATP in the firefly luciferin-luciferase BL reaction.