Countering the unresolved problems of maize anther culture such as the low induction frequency, great differences among the genotypes, the factors which effected the maize anther culture were centered on and the culture system was established which was fit for culturing high induction frequency of main anther culture.

The result showed that the induction frequency of pollen callus was irrelevant to the volume of culture containers,and highly related to the density of pollens.

Through analysis of primary resonance, superharmonic resonance and combination resonance, and therefore some frequency phenomena in laser breeding are explained.