1) Nuclear transfer and donor cells
核移植与供体细胞
2) Somatic cell nuclear transfer
体细胞核移植
1.
Influence of different parameters of electrical fusion and activation on development of porcine somatic cell nuclear transfer embryo;
不同电融合和激活参数对猪体细胞核移植胚胎发育的影响
2.
Firstly,reviewed the domestic and foreign progress of transgenic cattle produced by pronuclear microinjection and somatic cell nuclear transfer,Secondly,introduced the application of the new technologies,including gene targeting,RNAi and MMCT et al,in production of transgenic cattle and safe appraisal of transgenic cattle and its products.
综述了显微注射转基因牛和体细胞核移植转基因牛的国内外进展,介绍了基因打靶、RNA干扰和微细胞介导转染色体等新技术在转基因牛生产中的应用及转基因牛的安全评价,最后指出了当前转基因牛存在的问题及未来的发展前景。
3.
To examine the effects of somatic cell serial nuclear transfer (SCSNT) on buffalo somatic cell nuclear transfer (SCNT),SCSNT was conducted with the buffalo fetal cells as the donor cells.
05);这说明水牛体细胞核移植胚胎可被再次克隆而不降低其发育能力,水牛体细胞连续核移植是可行的。
3) somatic nuclear transfer
体细胞核移植
1.
Effects of ultraviolet irradiation on in vitro development of parthenogeneticand somatic nuclear transferred embryos in bovine;
紫外线照射对牛孤雌激活卵母细胞和体细胞核移植胚胎体外发育的影响
2.
Epigenetic modification related to somatic nuclear transfer
体细胞核移植诱导的表观遗传学变化
3.
So the current research was designed to study main items of pig somatic nuclear transfer systematically such as somatic donors, recipient oocyte, culture of cloned embryos and embryo transfer.
体细胞核移植为人们生产不引起免疫排斥以及无内源性病毒的猪提供了可能。
4) SCNT
体细胞核移植
1.
The oocytes having specific genetic background recovered by ovum pick up(OPU) technology, were used as recipient cells of somatic cell nuclear transfer(SCNT) to receive donor nuclei from different origins.
应用活体取卵(OPU)技术采集遗传背景清晰的卵母细胞作为受体细胞,与不同来源的供体细胞核进行体细胞核移植(SCNT)。
2.
The purpose of the study was to investigate in vitro development,quality and frozen tolerance of bovine somatic cell nuclear transfer(SCNT) embryos in two defined culture media(IVD101 and G1/G2).
通过考察牛体细胞核移植重构胚在无血清培养基(IVD101、G1/G2)条件下培养的囊胚发育率及其质量,从而评估无血清培养基支持牛体细胞核移植重构胚体外发育的能力。
3.
Later, pEBB was trasfected into bovine fetal fibroblasts and positive cells was selected to produce transgenetic cloned embryos by Somatic Cell NuclearTransfer(SCNT).
然后,将该载体转入胎牛成纤维细胞,得到的阳性细胞通过体细胞核移植(SCNT)来产生转hBD3基因的克隆囊胚。
5) porcine somatic cell nuclear transfer
猪体细胞核移植
1.
In order to determine the appropriate electric fusion parameters for porcine somatic cell nuclear transfer, four experiments were conducted in the present study, in which in vitro matured porcine oocytes were used as recipients and porcine granulosa cells were used as donors.
为确定猪体细胞核移植中适宜的电融合参数，本研究用体外成熟46小时的猪卵母细胞为核移植受体，猪颗粒细胞为核移植供体，共进行了四个实验。
6) nuclear transfer
体细胞核移植
1.
In comparison with the traditional method, this technique of somaticcell nuclear transfer has many advantages.
本实验研究了质粒浓度，脂质体浓度，脂质体-质粒复合物与细胞共孵育时间对GFP转染猪胎儿成纤维细胞效率的影响，通过G418的筛选，获得稳定表达绿色荧光蛋白基因的阳性细胞，并以此阳性细胞作为体细胞核移植的核供体，以体外成熟卵母细胞为核受体构建了绿色荧光蛋白转基因克隆猪胚胎，并对重构胚在体外和体内发育情况以及绿色荧光蛋白表达情况进行了跟踪研究。
补充资料：核移植
核移植
nuclear transfer
应用显微操作技术，将一个细胞的核移植到另一细胞中。利用此技术，可研究所移植核的发育潜能。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条