In observation group,the centrifugal puncture was performed by the great saphenous vein beside ankle,while in control group,the centripetal puncture was performed by superficial vein near the bottom of thu.

Objective: To explore the pplicatiom effect of the superfical vein reverse puncture near metacarpophalangeal joints to chronic discase patients of internal mdeicine.

Various puncture needles are usually repeatedly used in clinic.

Methods:The peripancreatic fluid from patients with severe acute pancreatitis(SAP) were obtained by ultrasound-guided percutaneous fine needle aspiration(FNA),the PCR assay was used to detect bacteria and compared with convention culture.

Objective To investigate the improved method to obtain enough pathological sample of myogenic tumor by curved linear array Endoscopic ultrasonography guided fine needle aspiration.

Purpose To investigate the expression of telomerase activity in tissues and corresponding fine needle aspiration(FNA) samples of thyroid neoplasms,and its clinical valuation.