1) restriction allele
限制性等位片段
2) restriction fragment length
限制性片段长度
1.
Genotyping of human platelet antigen system 5 by PCR restriction fragment length polymorphism;
限制性片段长度多态性分析人血小板抗原5系统基因型
2.
Methods RFLP(restriction fragment length polymorphism)detects and identifies Candida albicans,Candida tropicalis and Candida krusei in the simulation specimens comparing with conventional culture method.
方法对制备的白色念珠菌、热带念珠菌和克柔念珠菌感染的血液模拟标本采用常规血培养法和限制性片段长度多态性分析(RFLP)法进行检测和鉴定,最后进行结果分析。
3.
METHODS The genotypes of E-selectin were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 145 CHD cases,including 73 with acute coronary syndrome(ACS) patients and 72 cases with stable coronary heart disease(SCHD),and 144 cont.
采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,分析E-选择素基因型。
3) restriction fragment
限制片段
4) restriction fragment length polymorphism
限制性片段长度多态性
1.
Identification of 60 strains of clinical isolated Microsporum canis with restriction fragment length polymorphism
60株临床分离犬小孢子菌的聚合酶链反应-限制性片段长度多态性鉴定
2.
The research advance on the detection methods of beer-spoilage microorganisms is reviewed,including polymerase chain reaction,restriction fragment length polymorphism,immunity technology,nucleic acid hybrid-ization and,voltammetric biosensor.
内容包括聚合酶链式反应,限制性片段长度多态性,免疫学技术,核酸杂交,伏安型生物传感器。
3.
Methods HBV genotypes were determined by the restriction fragment length polymorphism analysis in 120 HBV DNA PCR positive patients infected with hepatitis B,including 17 asymptomatic.
方法选择宁夏回族HBVDNA阳性慢性乙肝病人共120例,其中病毒携带者(ASC)17例,慢性肝炎(CH)34例,肝硬化(LC)58例,肝细胞癌(HCC)11例,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测HBV基因型。
5) RFLP
限制性片段长度多态性
1.
Objective To establish a PCR-RFLP method for detection of CD26 mutation of hemoglobin E(HbE)gene.
目的建立PCR-RFLP(限制性片段长度多态性)法检测血红蛋白E(HbE)基因CD26突变。
2.
The point mutation of pre-C 1896-position was detected by PCR and restriction fragment length polymorphism (PCR-RFLP).
方法用荧光定量PCR检测64例HBeAg阴性HBV感染者血清HBVDNA,ELISA法检测preS1抗原,以错配聚合酶链反应结合限制性片段长度多态性(PCR-RFLP)分析HBV前C区1896位点的基因变异。
3.
Methods Genomic DNA was analyzed for the mutation allele in 100 healthy controls by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).
方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,对100名上海地区汉族健康者PAF-AH4号外显子275位点G/A位点的SNP进行了检测,计算其基因型和等位基因频率,并结合文献进行了不同种族间的分析比较。
6) Restriction fragment length polymorphisms
限制性片段长度多态性
1.
Identification of 4 Trichinella isolates by Restriction Fragment Length Polymorphisms analysis;
猪、犬旋毛虫DNA限制性片段长度多态性分析
2.
Methods:Restriction fragment length polymorphisms(RFLP)at PstI、SstI sites of ApoAI-CIII-AIV gene cluster were studied using polymerase chain reaction(PCR)in 161 patients with cholesterol gallstone and 94 healthy subjects from a Chinese population in Sichuan Province.
方法应用多聚酶链反应技术(PCR)对161例胆固醇结石病患者及94例正常人载脂蛋白(Apo)AI-CIII-AIV基因簇的限制性片段长度多态性(RFLP)进行研究,检测ApoAI-CIII-AIV基因位点PstI、SstI。
3.
Methods Lipoprotein lipase gene Hind Ⅲ polymorphism was studied using polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLP) in 103 type Ⅱb hyperlipoproteinemia patients and 129 healthy subjects from a population of Chinese Hans in Chengdu area.
方法 采用聚合酶链反应及限制性片段长度多态性方法对成都地区 10 3例 b型高脂蛋白血症患者及 12 9名血脂正常者脂蛋白脂酶基因 Hind 酶切位点的多态性及其与血脂、载脂蛋白水平的关联进行了研究。
补充资料：限制
分子式：
CAS号：
性质：一种保护细菌细胞不受外源DNA侵入的作用，通过限制性内切酶实现。该酶对双链DNA进行定点剪切。通常合成限制性内切酶的细胞由于还能合成识别同样位点的修饰酶而使细胞本身的DNA结构进行修饰，如甲基化而受到保护。当细菌受到噬菌体感染，而噬菌体DNA事先未经修饰，则这未经修饰的外来噬菌体DNA被切成小片段，再进一步为外切核酸酶所降解。未经修饰的噬菌体DNA引起感染的可能性比修饰过的噬菌体DNA要小几个数量级。
CAS号：
性质：一种保护细菌细胞不受外源DNA侵入的作用，通过限制性内切酶实现。该酶对双链DNA进行定点剪切。通常合成限制性内切酶的细胞由于还能合成识别同样位点的修饰酶而使细胞本身的DNA结构进行修饰，如甲基化而受到保护。当细菌受到噬菌体感染，而噬菌体DNA事先未经修饰，则这未经修饰的外来噬菌体DNA被切成小片段，再进一步为外切核酸酶所降解。未经修饰的噬菌体DNA引起感染的可能性比修饰过的噬菌体DNA要小几个数量级。
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