1) two-point cross
二点杂交
2) dot blotting
斑点杂交
1.
The dot blotting detection method was established to detect the V.
分别用胶原蛋白酶DNA探针和外膜蛋白DNA探针对实验室保种的溶藻弧菌以及鱼排分离的溶藻弧菌进行斑点杂交检测,检测结果表明,胶原蛋白酶基因探针与保种的溶藻弧菌以及鱼排分离的溶藻弧菌DNA产生较强的阳性反应,而与霍乱弧菌、哈氏弧菌和副溶血弧菌DNA等均无反应,表明该胶原蛋白酶DNA探针的特异性较强;而外膜蛋白基因探针与保种的溶藻弧菌以及鱼排分离的溶藻弧菌DNA产生较强的阳性反应,但与哈氏弧菌和副溶血弧菌DNA也出现了较强的阳性反应,与霍乱弧菌DNA则无反应,表明该外膜蛋白DNA探针的特异性较低。
3) Dot Blot
斑点杂交
1.
Evaluation or Polymerase Chain Reaction and Dot blot for HBV DNA Detection;
聚合酶链反应结合斑点杂交测定乙型肝炎病毒DNA的评价
2.
A quantitative dot blot method of detection for target gene relatively copy numbers in DNA sample;
DNA样品目的基因相对拷贝数的定量斑点杂交测定方法
3.
Methods Dot blot of chromatosome and plasmid DNA were performed in 2 Enterobacter cloacaes.
方法 分别提取细菌的染色体DNA和质粒DNA通过斑点杂交的方式 ,并将整合子聚合酶链反应(PCR)产物测序 ,来确定编码AmpC酶DHA 1型基因存在的位置。
4) Dot blot hybridization
斑点杂交
1.
Detection of IHHNV in shrimp by dot blot hybridization with digoxigenin labeled DNA probe by PCR;
PCR法制备地高辛标记DNA探针斑点杂交检测对虾传染性皮下及造血组织坏死病毒(IHHNV)
2.
anguillarum by dot blot hybridization.
选取2株鳗弧菌和8株与鳗弧菌亲缘关系非常接近的常见水产动物致病弧菌,通过斑点杂交法对制备的探针进行特异性检测,结果显示该探针对鳗弧菌杂交阳性,而与弧菌属的其它8株细菌均无交叉反应。
3.
Then white spot syndrome virus was detected by PCR and dot blot hybridization before and after soaking Brachionus plicatilis.
对褶皱臂尾轮虫进行白斑综合征病毒(WSSV)攻毒,然后将攻毒前后的轮虫进行PCR及核酸探针斑点杂交检测。
5) dot blot hybridization
点杂交
1.
Identification of hEPO gene integration into chicken genome by dot blot hybridization;
用点杂交法检测人促红细胞生成素(hEPO)的整合
6) dot hybridization
斑点杂交
1.
Detection of -Sugarcane mosaic virus- by dot hybridization and RT PCR technique;
利用斑点杂交法和RT-PCR技术检测甘蔗花叶病毒
2.
(Methods The expressions) of IGF-ⅠmRNA of WI-38 and Hela cell lines in silver nanomaterial group, rare-earth nanomaterial group and control group were tested by in situ hybridization, dot hybridization and quantitative analysis technique.
②方法通过原位杂交、斑点杂交及定量分析技术分别检测银系纳米材料组、稀土纳米材料组与对照组WI-38及Hela细胞IGF-ⅠmRNA表达。
3.
The positive clones were selected by dot hybridization with Dig-labeled probe.
利用地高辛标记探针进行斑点杂交检测 ,筛选出阳性克隆 ,拟用该克隆转化马铃薯细胞 ,研制新型轮状病毒疫苗。
补充资料：斑点
在一种颜色的物体表面上显露出来的别种颜色的点子。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条