Based on the observation of polymer spherical crystal configuration with polarization microscope,the specific application of this system is also introduced.

The LC configuration was determined with self-made polarization microscope.

According to the anisotropic character of yak hair,its red-blue ratio of interfer-ence colour after each bleaching treatment is obsered by a polarization microscope with red(660nm)and blue(470nm)filter plates,It is found that there exists a relation between the red-blue ratio of interference colour of bleached yak hair with the contents of FeSO4 and H2O2 in theoxidizing bath.

Sum modified-Laplacian(SML) operator has been proposed as focusing measuration to measure the relative sharpness of image sequence that captured from microscope at different distance to locate the height of surface of wear debris, and reconstruct the 3D image of wear-debris.