Study on enzymic hydrolysis condition of soybean isoflavone glycoside;

Hydrolysis of soybean isoflavone glycoside;

The study worked a new method that experiment adopted Aspergillus niger as experimnetal stecies, which secrete β-glucosidase, to produce the soybean isoflavone glycoside by solid fermentation.

The article worked a new method that we adopted Aspergillus niger as our stecies, which secrete β-glucosidase,to produce the soybean isoflavone glycoside by solid fermentation.

Results One isoflavone glycoside and three isoflavones were purified anddetermined as 8-methylretusin-7-O-β-D-glucopyranoside (Ⅳ), retusin 8-methyl ether ( Ⅱ ), 4 , 7, 8-trimethoxyisoflavone ( Ⅲ ), and afrormosin ( Ⅵ ).

One new flavone and one new isoflavone glycoside were isolated along with twenty-six known related compounds from the rhizome of Belamcanda chinensis (Iridaceae) , and their structures were characterized as 5,4′-dihydroxy-6,7-methylenedioxy-3′-methoxyflavone, and 3′,5′-dimethoxy irisolone-4′-O-β-D-glucoside on the basis of spectroscopic methods and chemical evidence.

Study on soybean isoflavone-glycosidase fermented by Aspergillus oryzae;

By means of orthogonal test,the condition for hydrolyzing soybean isoflavones glycoside into isoflavones aglycon was optimized as follows:methanol hydrochloride concentration 2mol/L,temperature 80℃,time 60min.