Methods According to theory of specific binding of antigen and antibody,at first the anti-A monoclonal antibody(MA) and anti-B MA were labeled with the fluorescent,then fluorescent-labeled antibodies(FLA) were bound with corresponding biological material(such as bloodstain) in the optimum condition,finally the ABO blood type of bloodstain was determined under microscope fluore.

This experiment was carried out in order to compare the application of Tf test kit with Hb test kit on controlled bloodstains by using the immunochromatography assay.