Objective To determine the characteristics of Neisseria meningitides (Nm) in Guangdong Province in different epidemic periods, especially after late 1980s.

Objective: To establish a PCR optimal system for detecting Neisseria meningitides, and discuss multiple factors which affect this PCR assay.

[Conclusion] The real-time PCR assay is rapid, sensitive and specific assay for detection of Neisseria meningitides, and could be a valuable method for further understanding of the epidemiology of meningococcal infection.

Study on the genotype and assessment for Neisseria meningitidis;

Investigation on Carriage of Neisseria meningitidis and Analysis of Serogroup C Strains by PFGE in Jiangxi Province,2005;

Detection of Neisseria meningitidis from Cerebrospinal Fluid by Polymerase Chain Reaction;

Rapid identification of Neisseria meningitides by γ-glutamyltransferase

Application of real-time PCR for detection of Neisseria meningitides

Methods Neisseria meningitides was detected from cerebrospinal fluid and serum samples of suspected epidemic cerebrospinal meningitis cases.

Objective To prepare MenA PS-EA conjugate and develop an ELISA kit for IgG against capsular polysaccharide of Nesseria meningitidis serogroup A using the MenA PS-EA as coating antigen.