1) clip
[英][klɪp] [美][klɪp]
夹,小夹
4) small hairpin RNA
小发夹RNA
1.
Now there are four main kinds of RNA interference libraries with different construction approaches and molecular form,chemically synthesized small interference RNA(siRNA) libraries,chemically synthesized small hairpin RNA(shRNA) libraries,enzymatically prepared siRNA libraries and enzymatically prepared shRNA libraries.
不同种类的RNA干扰库已经被用于基因功能的研究之中,根据其构建方式和分子形式的不同,可将RNA干扰库分为4种类型,即化学合成的小干扰RNA(siRNA)库、化学合成的小发夹RNA(shRNA)表达库、酶切法构建的siRNA库和酶切法构建的shRNA表达库。
2.
[Methods] A shRNA expressed vector that expresses the specific small hairpin RNA targeting TRAP mRNA was constructed and transfected into SGC-7901 cells, and the stably expressing shRNA cells were selected by G418 and continuously cultured in half the antibiotic concentration for 3 months.
目的探讨稳定转染靶向端粒酶调节相关基因TRAP的小发夹RNA对胃癌细胞株SGC-7901的长效影响。
3.
[Methods] Plasmid vectors expressing small hairpin RNA (shRNA) targeting at cyclin D1 gene were constructed and transfected into K562 cells mediated by chitosan.
[方法]体外构建靶向cyclin D1基因的小发夹RNA(shRNA)表达质粒,通过壳聚糖介导转染K562细胞,Western blot分析检测转染前后cyclin D1蛋白表达变化;集落形成实验检测细胞增殖能力;流式细胞仪检测细胞周期分布及凋亡情况。
5) shRNA
小发夹RNA
1.
Construction Expressing Vector of shRNA Inhibiting Cyclin D1 Gene Expression by RNA Interfering;
抑制cyclin D1基因小发夹RNA(shRNA)表达质粒的构建
2.
[Methods] The 60nt nucleiotide sequence encoded targeting EBNA2 shRNA was cloned into a retroviral vector pSUPER.
[方法]用DNA重组技术将60nt能转录产生靶向EBNA2小发夹RNA(smallhairpin RNA,shRNA)的寡核苷酸序列定向插入逆转录病毒载体pSUPER。
3.
Objective:To construct and identify the small hairpin RNA(shRNA) expression vector for Smad 4/DPC 4 gene.
目的:构建Smad4基因小发夹RNA(shRNA)表达载体。
6) Small splint
小夹板
1.
Small splint immobilization for treatment of earthquake casualties
地震伤员小夹板固定治疗
2.
For a long time,there is a firm problem about the application of a small splint that adjustment of cincture’s tightness degree is decided by the doctor s feeling and experience.
医用小夹板是四肢骨折康复治疗的重要器械。
补充资料:夹
夹
夹 古代中医眼科手术器械名称。见《目科正宗》。多用老竹制成。用以治疗睑废、倒睫拳毛等。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条