Methods Forty-eight samples from patients with ovarian tumor at different clinical stages and 8 from normal ovaries were examined for NM23-H1B mRNA expression by using RT-PCR, northern blot and in situ hybridization.

METHODS: The validity and reliability of the results gotten by serial analysis of gene expression method was verified by RT-PCR and Northern blot.

Methods: Total RNA was isolated from peripheral leukocytes of healthy men and women, AR mRNA was determined by RT PCR and Northern blot.

Extraction and Yield of Total RNA in Cochleas of Normal Rats and Long-Term Sodium Salicylate Treated Rats;

By using the modified CTAB method, total RNA was isolated from Camellia oleifera leaves rich in such secondary products as polyphones and polymeric carbohydrates, and then mRNA was isolated and purified through double selections using oligo(dT) cellulose.

Isolation of Total RNA from Rat Liver Tissue by Improved Trizol Method;

Total RNA Extraction from the Bulbs of Fritillaria thunbergii Miq.;

In vitro cultivation and identification of dendritic cells transfected with total RNA from Ewing sarcoma cells;

Advances in the small RNA of plant;

Eukaryotes produce various types of 19-30 nt small RNAs,which act as guides to the regulation of gene expressions,such as mRNA degradation and translational repression.

The researches on small RNAs used to focus mainly on mammals in the past,but with the development of bioinformatics and molecular biology,some small RNAs have been found in prokaryotes.

Effects of microRNA miR-181a on gene expression profiles of K562 cells;

Gene transfection and expression of murine liver-specific microRNA in HeLa cell line;

Construction and evaluation of eukaryotic expression vector of mouse liver-specific microRNA;