Construction of eukaryotic expression vector of siRNA specific for osteopontin and characterization of its efficiency in GC9811 gastric carcinoma cell line;

Objective: To construct the eukaryotic expression vector pcDNA3.

Construction and identification of bicistronic eukaryotic expression vector pVAX1-IRES for gene coexpression;

Eukaryotic expression and identification of human pSecTag2B-CD226 fusion protein;

Construction and expression of HSC70 gene eukaryotic expression plasmid;

Construction of eucaryotic expression vector of bioactive fragment gene of human bactericidal/permeability increasing protein and its expression in COS-7 cells;

Objective To construct the recombinant plasmid of Eucaryotic expression containing Tp92 Gene from Treponema pallidum,and transfect it into Hela cells to express the outer membrane protein.

In the first part of the research, in combination of the common character between the eukaryotic and prokaryotic expression system, we desighed and synthesized the fusion gene, cascade connection of three marine antimicrobial peptide, which is coined as Moronecidin-Hepcidin-Pleurocidin.

Cloning and eukaryotic expression of the gene encoding the new antigen Mtb8.4 of Mycobacterium tuberculosis;

Selection and Identification of Strains Producing Fibrinolytic Enzyme from Lobster Sauce and Stuides on Expression of Fibrinolytic Enzyme Gene in Eukarya;

Methods The ECV-304 cells were infected by Eukaryotic Expression plasmid pcDNA3.