2) suppression subtractive
抑制性差减
1.
Construction of suppression subtractive libraries of brain tissue from apoplectic stroke rats and normal rats;
卒中大鼠脑组织抑制性差减文库的构建
3) suppression subtractive hybridization
抑制性差减杂交
1.
Suppression subtractive hybridization(SSHs) was performed to distinguish the differentially expressed genes.
取培养48h的2组细胞提取总RNA后分离mRNA,应用抑制性差减杂交法分离差异表达基因,并连接于pGEM TEasyVector,转化大肠杆菌JM109,构成差减cDNA文库,经蓝白斑筛选后挑选白色菌落,提取质粒,序列测定后进行同源性比较分析。
2.
A systematic study was initiated to identify differentially expressed genes in the process of sex reversal of the grouper by using suppression subtractive hybridization (SSH) technique.
应用抑制性差减杂交 (SSH)技术构建了石斑鱼性反转前后性腺组织的SMARTcDNA文库及其cDNA差减文库 ,从中随机挑取 12 0 0个克隆进行了PCR和斑点杂交筛选 ,得到 12 0个差异表达cDNA片段 。
3.
Objective: To Construct a subtracted cDNA library of hyperlipidemia sensitive rabbits′liver by suppression subtractive hybridization(SSH).
结论:用抑制性差减杂交及T/A克隆技术成功构建了高脂血症敏感兔差异表达基因差减cDNA文库。
4) Suppression subtractive hybridization (SSH)
抑制性差减杂交
1.
Suppression subtractive hybridization (SSH) was used to identification of differentially expressed genes in chicken kidney during avian influenza virus infection.
利用抑制性差减杂交技术(suppression subtractive hybridization,SSH)成功构建了禽流感病毒感染和正常对照鸡肾组织差异表达的双向差减cDNA文库,通过斑点杂交筛选到包括干扰素刺激基因(ISG12)、淋巴细胞抗原复合体基因(LY6E)、基质Gla蛋白基因 (MGP)、溶菌酶基因、IFN诱导的跨膜蛋白基因及一个没有任何同源信息的EST等6个表达上调基因;KIAA1259、谷光苷肽转移酶基因(GST)、酒精脱氢酶基因(ADH1)、突触结合蛋白基因(SytVx)、葡萄糖6磷酸酶催化哑基基因(G6PC)、钠/二羧酸转运蛋白基因(SDCT)、 MGC4504基因、MGC69686基因和一个没有任何同源信息的EST等9个表达下调基因。
5) SSH
抑制性差减杂交
1.
Construction of SSH library from haemocyte of variously colored abalone challenged with bacteria and differential expression analysis of macrophage expressed protein;
细菌攻毒杂色鲍血淋巴细胞抑制性差减杂交文库构建及巨噬细胞表达蛋白cDNA的克隆与差异表达
6) Suppression subtractive hybridization(SSH)
抑制性差减杂交
1.
In order to find putative virulent genes of Riemerella anatipestifer,suppression subtractive hybridization(SSH)was used between virulent R.
为寻找鸭疫里氏杆菌(Riemerella anatipestifer,RA)可能的毒力基因,以鸭疫里氏杆菌2型(RA2)毒力株RAF2及弱毒力株LY-58为研究对象,通过抑制性差减杂交(SSH)试验,从RAF2株中获得14个特异性差异基因片段。
补充资料:连续性与非连续性(见间断性与不间断性)
连续性与非连续性(见间断性与不间断性)
continuity and discontinuity
11an父ux泊g四f“山。麻以角g、.连续性与非连续性(c。nt,n琳t:nuity一)_见间断性与不间断性。and diseo红ti-
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条