The results indicated that with double PCR and double blastomeres template,we could success to do sex identification on 8-cell embryos of mouse,and the male embryos had a better developmental potentiality than female in vitro.

The early developmental potential of these oocytes were analyzed after parthenogenetic activation and the dynamic of damage and reconstruction of the spindle were detected by confocal microscopy.

Objective To detect the gene expression level of mitochondrial genome in single human oocyte or embryo,and to explore the relationship between the gene expression level of mtDNA and oocyte developmental potential.