1) lap splice
重叠拼接
3) splicing by overlapping extention
重叠延伸拼接
4) gene splicing by overlap extension
重叠区扩增基因拼接法
1.
Construction of middle fragment deletion mutant with improved gene splicing by overlap extension;
用改进的重叠区扩增基因拼接法构建中间缺失突变体
2.
The fragments of HBV genotype C were mutated by using gene splicing by overlap extension (gene SOEing).
方法通过不同基因型HBV全基因组的序列比对,选取6个碱基位点作为分型位点,应用重叠区扩增基因拼接法(SOEing法)对C型HBV片段进行点突变。
5) SOEing
重叠区扩增基因拼接法
1.
Gene splicing by overlap extension(SOEing)method was used to mutate the 228 base of HBV genotype C by gene,and the sequences were confirmed by sequencing.
结论重叠区扩增基因拼接法(SOEing)是一种简便引入点突变的方法,可以应用此法制备HBV基因分型芯片检测基因片段序列。
2.
To clone and express the gene recombinant slp-man in Escherichia coli JM109,the strong promoter of Bacillus thuringiensis CTC surface layer protein and the β-mannanase gene ORF of Erwinia carotovora CXJZ95-198 were ligated via gene splicing by overlap extension(SOEing) .
为构建β-甘露聚糖酶基因高效表达体系,采用重叠区扩增基因拼接法将苏云金芽孢杆菌(Bacillus thuringiensis)CTC S-层蛋白启动子和欧文氏杆菌(Erwinia carotovora)CXJZ95-198 β-甘露聚糖酶基因完整开放阅读框定向连接,得到基因拼接体slp-man。
6) gene splicing by overlapping extensionPCR
基因重叠延伸拼接PCR
补充资料：信使拼接
分子式：
CAS号：
性质：对来自高等生物的基因的一次转录的编辑，通过核酶(ribozyme)切除非编码的插入序列。信使拼接后留下了连续的编码序列，以此规定所编码的蛋白质。
CAS号：
性质：对来自高等生物的基因的一次转录的编辑，通过核酶(ribozyme)切除非编码的插入序列。信使拼接后留下了连续的编码序列，以此规定所编码的蛋白质。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条