In the anterior hypothalamus,a concomitant increase in the basal extracellular 5-HT levels was measured by using in vivo microdialysis in awake rats on Day 4 following KLH administration.

Coupled with high performance liquid chromatograghy (HPLC) and radioimmunoassay (RIA), brain dialysis is allowed to determine the extracellular changes of many neurotransmitters in the brain, such as acetylcholine, noradrenaline, dopamine, 5 HT and their metabolites, free amino acids, small peptides.

By searching the relevant literature over the past decade, the brain microdialysis technique to the profiles, the principle of the brain microdialysis probe and its application are reviewed, hope to be able to engaging in the study of pharmacy workers to provide relevant reference.