In order to confirm the existence of rhodopsin in cerebrospinal fluid-contacting neuron and the effect of illumination on it,pineal gland of rat was immediately taken after it was killed and examined the existence of rhodopsin with fluorescence microscope by immunohistochemical method and confirmed the effect of light on rhodopsin in the pineal gland by electrophysiological method.

Aim To observe the neuron nitric oxide synthase(nNOS) expression in the distal cerebrospinal fluid contacting neurons(CSF-CNs) of rat brain parenchyma, and investigate the role of CSF-CNs in the development of morphine dependence and withdrawal.

By a doubling labelled method combining the retrograde tracing of CB HRP and the immunocytochemical technique Fos protein, the expression of Fos protein in the cerebrospinal fluid contacting neurons (csf cn) of dorsal raphe nucleus(DR)was observed following gastro intestinal noxious stimulation .

Coexistence of nitric oxide synthase with vasopressin in cerebrospinal fluid-contacting neurons of the third ventricle in spontaneously hypertensive rat;

NADPH-diaphorase histochemistry method was used to investigate the distribution and morphology of nitric oxide synthase (NOS) positive cerebrospinal fluid-contacting neurons (CSFCN) in the periventricular region of the third ventricle in the rat.

Comparative study on staining methods of CB-HRP for visualization of CSF-contacting neurons in electron microscopy;

Objective The ultrastructure of the distal CSF-contacting neurons in the dorsal raphe and the relationships with their surrounding tissues were observed and a new morphological evidence for their functional significance was offered.

By Using a combined method of CB-HRP tracing with immunocytochemistry, chemical natures of CSF-contacting neurons in the dorsal raphe nucleus were studied.