1) HPMC(human peripheral mononuclear cell)
人类外周血单核细胞
2) human PBMC
人外周血单个核细胞
1.
METHODS:The human PBMCs were stimulated by CpG ODN and/or immunoregulatory ODN.
方法:利用含有CpG基序的免疫刺激性寡聚脱氧核苷酸(CpG ODN)作为刺激物,将人外周血单个核细胞(PBMC)作为研究对象,分别以细胞的增殖程度和刺激上清的抗病毒活性作为检测指标,优化各项实验条件,综合评定寡聚脱氧核苷酸的免疫调节活性。
2.
METHODS: Effects of various concentrations of DON on TAP-1 expression of in vitro cultured human PBMCs and the dose-effect relationship were analyzed by flow cytometry (FCM) and semi-quantitative RT-PCR at the protein and mRNA levels.
目的: 探讨脱氧雪腐镰刀菌烯醇 (DON)对人外周血单个核细胞 (PBMC)中TAP- 1表达的影响。
3) human peripheral blood mononuclear cell
人外周血单个核细胞
1.
Effects of sterigmatocystin on HLA-Ⅰexpression of human peripheral blood mononuclear cells in vitro;
杂色曲霉素对人外周血单个核细胞表面HLA-Ⅰ分子表达的影响
2.
To explore the effects of fumonisin (FB1) on TAP-1 (transporter associated with antigen processing) expression of human peripheral blood mononuclear cells (hPBMC) in vitro.
探讨伏马菌素B1(FB1)对体外培养的人外周血单个核细胞(hPBMC)抗原加工相关转运子(TAP-1)表达的影响。
3.
To explore the effects of deoxynivalenol (DON) on low molecular weight polypeptide-2 (LMP- 2) expression of human peripheral blood mononuclear cells.
探讨脱氧雪腐镰刀菌烯醇(DON)对人外周血单个核细胞参与抗原呈递的低分子量蛋白酶体-2(LMP-2)表达的影响。
4) human peripheral blood mononuclear cells
人外周血单个核细胞
1.
The effects of human peripheral blood mononuclear cells on the proliferation and differentiation of human periodontal ligament fibroblast cells;
人外周血单个核细胞对人牙周膜成纤维细胞增殖和分化的影响
2.
Objective To investigate the efficacy of romurtide(RM),the effective component of Bacillus Calmette?Guerin (BCG) in cytokine production by human peripheral blood mononuclear cells (HPBMCs).
目的 :探讨卡介苗 (BCG)细胞壁有效成分罗莫肽 (RM)对人外周血单个核细胞 (HPBMCs)表达细胞因子的刺激作用。
3.
Method Flowcytometry was carried to analyze the FasL expression of human peripheral blood mononuclear cells (HPBMC) transfected with recombinant retrovirus vector which was able to transcript anti sense RNA of FasL.
方法 :获得FasL反义RNA重组逆转录病毒 ,调整感染滴度后转染活化人外周血单个核细胞 (HPBMC)。
5) human peripheral blood mononuclear cells (HPBMCs)
人外周血单个核细胞(HPBMCs)
6) mononuclear cell
人外周血单核细胞
1.
Effects of deoxynivalenol on proliferation and tumor necrosis factor-α secretion of human peripheral blood mononuclear cells in vitro;
脱氧雪腐镰刀菌烯醇对体外培养人外周血单核细胞增殖及肿瘤坏死因子-α分泌的影响
2.
The effect of ST on interleukin 2 (IL 2) secretion of human peripheral blood mononuclear cells (HPBMc) in vitro was determined with enzyme linked immunosorbent assay (ELISA) method to explore its putative effects on human immune function.
为探讨杂色曲霉素 (ST)对人体免疫机能的影响 ,采用双抗体夹心ELISA方法对ST作用后人外周血单核细胞 (HPBMc)培养上清液中白血细胞介素Ⅱ (IL 2 )的分泌水平进行了检测。
补充资料：人体外周血淋巴细胞培养
人体外周血淋巴细胞培养
诊法。为 检查患者染色体最简便的细胞培养技术。先于无菌条件下制备RPMI1640 或TC199等常用培养基，加适量小牛血清和PHA(植物血球凝集 素)，夏季操作时最好加适量青、链霉素，调pH值为7.0～7.2左右，4℃条件下保存6个月 。检测时，以500IU／ml肝素湿润注射器，抽取患者静脉血1.5～2ml(或取耳垂、指尖或足 跟等处微量血)，每份培养基(5ml)内种血0.2～0.3ml，37℃温箱内静置培养72小时，收获 前3～4小时加秋水仙素(或秋水仙胺)，最终浓度为0.02μg／ml，使细胞分裂终止于中期。 取出培养物行离心、低渗和固定等一系列细胞学操作获得染色体标本，以Giemsa染色后镜检 ，可作染色体标本的普通分析。现各实验室一般将染色体行G显带或必要的其他多种显带， 以较精确地对染色体标本进行分析。
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参考词条