Bluetongue virus\%(BTV)HbC strain and BTV 10 (the international standard strain) were respectively inoculated onto the different species monolayer cells such as Vero cell (monkey renal cell), C\-6 cell (mouse neuroglioma cell) and Hela cell (human cervical cancer cell).

There are 24 serotypes of bluetongue virus(BTV) .

Objective:To establish a high sensitive bio-bar codes assay method for detecting bluetongue virus(BTV) VP7 protein.

Some Molecular Biological Characters of a Cyanophage First Isolated in China;

Production and Biological Characteristics of Monoclonal Antibodies Against Bluetongue virus

A gene chip was developed by preparing highly conserved DNA fragments for five animal infectious disease viruses ( Foot and mouth disease virus, Vesicular stomatitis virus, Bluetongue virus, Epizootic haemorrhagic disease virus of deer, Akabane disease virus ) using molecular cloning methods, and the DNA fragments were spotted on to specially treated glass slides.

The plasmids were developed by means of using respectively high conservative DNA fragments of vesicular stomatitis virus, bluetongue virus, foot-and-mouth disease virus, classical swine fever virus, bovine viral diarrhea virus, epizootic haemorrhagic disease virus of deer and Akabane disease virus.

Using BALB/c mice immunized with purified VP 7 of Blue tongue virus 11 (BTV 11 ) and lymphocyte hybridoma technique and indirect ELISA screening, three strains of hybridoma cells secreting the group specifical monoclonal antibodies (McAb) to VP 7 of BTV 11 were obtained, which were designated as C 12 D 9 , B 4F 3, and E 1A 7.