The genes encoding the heavy and light chain variable region (VH and Vk) were combined to the human Cr3 and Ck genes respectively to construct the murine-human chimeric antibody genes.

For construction of anti-CD3 human/murine chimeric antibody genes, a selective first-strand cDNA synthesis from mRNA or RNA of murine McAb HIT3a was performed using murine Ig constant region primers, and then cDNA of heavy and light chain variable domains of murine immunoglobulin were amplified by PCR using a set of degenerated oligonucleotide primers.

Immunogenicity of chimeric gene vaccine Mtb8.4/hIL12;

Construction and Identification of DNA vaccine containing chimeric gene gag-gp120 of HIV-1;

Construction of recombinant baculovirus vector of HIV-1 gag-gp120 chimeric gene;