Objective: to explore whether there’re targeting effects of folate-penicillin G amidase conjugate (F-PGA) on folate receptor positive cells and solid tumors, and to investigate its pharmacokinetic parameters in vivo.

As a novel family of DNA-target molecules, 8-Oxo-8H-acenaphtho[l,2-b]pyrrole-9-carbonitrile and its derivatives (A1-A5), their interaction with DNA and antitumor abilities are evaluated by agarose gel electrophoresis, fluorescence spectrum, DNA melt curves, cytotoxicity experience.