Four kinds of multi-determinant artificial antigen was prepared by those haptens conjugating to the carrier protein(BSA,HSA,KLH)in turn.

Synthesis and characterization of artificial antigen for α-naphthol and β-naphthol;

Study of dicyclohexyl phthalate on preparation and characterization of artificial antigen;

Synthesis and identification of artificial antigens of 2,4-dichlorophenol;

Artificial antigens which were synthesized conjugating PTX-PDP to KLH-SH were used to immunized Balb/c mice to obtain polyclonal antibodies.

The synthesis on artificial antigens of pesticides directly influences the quality of the antibodies,and is critical in the format of particular and sensitive immunology assay.

The progress of carrier selection,design of hapten(emphasizing hapten-carrier coupling position,attachment of connecting arm),methods for the synthesis of hapten-protein conjugation,purification and identification of artificial antigens were introduced in this paper.

Synthesis and Identification of the Antigens for the Organophosphorus Insecticide Chlorpyrifos;

Synthesis and identification of antigenic conjugates of podophyllotoxin;

Study on an enzyme-linked immunosorbent assay kit for clenbuterol hydrchloride --Ⅰ preparation of clenbuterol immunogen and antibody;

Cyclosproine A reacted with 4 benzoylbenzoic acid under ultraviolet radiation to yield the conjugate of CsA BBa, the conjugate was coupled to polylysine (PL) and then the immunogen of CsA was obtained.

We got three immunogen—BP-BSA(physiology),AP-BSA(physiology) and AP-BSA(glutaraldehyde),they were identified by ultra violet scan,infrared spectrogram and SDS-PAGE electrooresis,and were found the rate of integration was 11∶1~12∶1.