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1)  counter-selecting
减差筛选
2)  subtraction biopanning
差减筛选
1.
Methods Osteosarcoma cells os-732 were used as the target cells and osteoblasts as the absorber cells for subtraction biopanning from a 12-mer peptide phage-display library.
方法以骨肉瘤细胞os-732为靶细胞,成骨细胞为吸附细胞对噬菌体12肽库进行差减筛选,用细胞ELISA、免疫组化鉴定阳性噬菌体克隆并测序。
2.
Methods With the LoVo cells as the target cells and human normal large intestinal mucosal epithelial cells as the absorber cells for subtraction biopanning from a c7c phage-display peptide library, the positive phage clones were identified by enzyme-linked immunosorbent assay (ELISA) and immuno.
方法以大肠癌LoVo细胞株作为靶细胞,人正常结肠黏膜上皮细胞为吸附细胞对噬菌体展示环七肽库进行差减筛选,采用细胞ELISA与免疫荧光检测鉴定噬菌体阳性克隆并测序。
3)  screening by PCR substration
PCR差减筛选
4)  subtractive screening
消减筛选
1.
METHODS:Whole-cell subtractive screening with phage display 7TM peptide library was performed on HepG2 cells and normal L-02 cells.
方法:以L-02为阴性消减细胞,HepG2为靶细胞,对噬菌体随机七肽库进行4轮消减筛选。
5)  subtract biopanning
减性筛选
6)  differential screening
差异筛选
1.
The subtractive differential screening was used to clone a novel mouse gene Bsg6(brain specific gene 6) expressed specifically in E9.
Bsg6(brain specific gene 6)是用消减差异筛选的方法克隆的小鼠头部特异表达新基因。
2.
Objective To explore the possibility of differential screening cDNA fragment library by means of colony PCR.
目的探索菌落PCR差异筛选cDNA片段文库的可行性。
补充资料:减缘减行
【减缘减行】
 (术语)缘者,指上界四谛与上二界四谛之八谛。行者,指欲界四谛下十六行相与上二界四谛下十六行相之三十二行相。自声闻乘四善根之初至忍法之上忍,连环普观此上下八谛之三十二行相,同自中忍之位,一行减之,遂至留苦或道等之一行相,谓之减行,每减其四行相自减一谛,故谓之减缘。其所以减之者,泛观上下八谛之三十二行相,观智浮漫而不猛利,故自中忍以后渐渐狭其观境,以养成猛利之观智,遂为发真无漏智之算引也。上下八谛之三十二行相,第一回自第一观至第三十一,因而减去第三十二即上界道谛下出之一行相,第二回更自第一观至第三十,因而减去第三十一即上界道谛下行之一行相。如此每一周自下逆次减一行相,终至减第一即欲界苦谛下苦之一行相。即减去者,三十一行相也。减此三十一行相中,每第四周减一谛,故谓之减缘,减于他三周之行相,谓之减行。即减缘七周,减行二十四周,合于三十一周减去三十一行也。第四周谓之减缘者,上下八谛,为所缘之境,三十二行相,为对于此之能缘观解,故减四行相即减所缘之境一谛也。因而逆次减去,每至第四行相,不谓为减行,特附以所谓减缘即减谛之名耳。(参见:行相)
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