1) thiophilic chromatography
亲硫层析
1.
Combining the thiophilic chromatography and mixed mode adsorption,new adsorbent with thiophilic mixed-mode ligands were developed and used for antibody separation.
本文结合亲硫层析和混合模式吸附,制备新型的亲硫性混合模式吸附剂,并用于抗体分离。
2) Affinity chromatography
亲和层析
1.
Screening the inhibitors for PrA from Ganoderma lucidum by immobilized enzyme affinity chromatography;
固定化亲和层析分离灵芝发酵液蛋白酶A抑制剂
2.
Purification of Glutathione (GSH) by metal-chelated affinity chromatography;
金属螯合亲和层析分离纯化谷胱甘肽(GSH)初探
3.
A new technology of isolation and purification:affinity chromatography;
分离纯化新技术亲和层析
3) Immunoaffinity chromatography
亲和层析
1.
Purification of NGF from Naja naja atra Cantor venom by immunoaffinity chromatography WEI Chuan-bao, HUANG Ya-nan;
舟山眼镜蛇毒神经生长因子的亲和层析分离
2.
Immunoaffinity chromatography was used to purify rhI.
用BmNPV/IGF 1感染家蚕幼虫 ,提取家蚕血淋巴液 ,采用亲和层析法纯化rhIGF 1,ELISA法测定rhIGF 1含量 ,用SDS PAGE和Western blot分析鉴定rhIGF 1纯度及免疫学活性 ,MTT法观察其对MCF 7细胞增殖的影响。
4) Affinity chromatograph
亲和层析
1.
coli BL21and myostatin fusion protein was purified by affinity chromatography.
为研究猪肌生成抑制素(myostatin,MSTN)蛋白的功能及为制备此蛋白的单克隆抗体提供材料,将原核表达菌种MSTN-PGEX-4T-1-BL21进行融合表达,用亲和层析的方法对GST-MSTN和MSTN C-端成熟蛋白进行分离纯化。
2.
The development of affinity chromatography technique is very fast in recent years,remarkable ac-complishments have been achieved in the separation and purification of biomolecules and organisms as well as in studying the interactions between biomolecules.
近几十年来,亲和层析技术发展十分迅速,广泛应用于生物分子(如结合蛋白、酶、抑制剂、抗原、抗体、激素、激素受体、糖蛋白、核酸及多糖类等)及组织(如细胞、细胞器、病毒等)的分离和纯化,是蛋白质组学研究中重要的技术之一。
3.
Based on their specificity, Sepharose CL-6B was adopted in this study as affinity chromatography to separate the S-type lectin in annelid from the earthworm, in which EDTA-MEPBS (2 mmol/L EDTA, 4 mmol/Lβ—mercaptoethanol, 150 mmol/L NaC1, 20 mmol/L phosphate, pH7.
根据其性质,本研究采用惰性分子筛填料Sepharose CL-6B作为亲和层析介质对蚯蚓S型凝集素进行了纯化。
5) Affinity chromatography
亲合层析
1.
Objective To prepare a more stable divalent β2m-HLA-A2/IgG1 molecule by covalent association of β2m with the HLA-A2 heavy chain,which is expected to be more stable during the purification by staptylococcal protein A(SPA) affinity chromatography.
目的HLA-A2/IgG1二聚体经蛋白A(SPA)亲合层析柱浓缩纯化时容易发生β2 m解离,为了解决这个问题,研究构建与表达β2 m-HLA-A2/IgG1融合蛋白。
6) Chromatography
[英][,krəumə'tɔgrəfi] [美][,kromə'tɑgrəfɪ]
亲和层析
1.
Bind Resin Chromatography under denaturing condition and dialyzed for renaturation, and then were analyzed with SDS-PAGE, West.
目的探讨通过金属螯合亲和层析的方法获得高纯度的人内皮细胞高表达脂多糖相关因子1(EOLA1)蛋白的可行性。
2.
The fusion protein was purified by chromatography Glutathione Sepharose 4B and digested by 3C protease.
用glutathioneSepharose 4B亲和层析柱纯化表达产物后 ,以重组 3C蛋白酶对融合蛋白进行解离 ,SDS PAGE对表达及纯化产物进行鉴定。
补充资料:5-硫基-3-苯基-2-硫-1,3,4-硫代二唑,钾盐
CAS: 17654-88-5
分子式: C8H6N2S3
中文名称: 铋试剂II;3-苯基-1,3,4-噻二唑亚基-2,5-二硫酮;5-硫基-3-苯基-2-硫-1,3,4-硫代二唑,钾盐;铋试剂 II
英文名称: BismuthiolII;5-Mercapto-3-phenyl-2-thio-1,3,4-thiodiazole, potassium salt;4-thiadiazole-2-thione, 5-mercapto-3-phenyl-2h-3;4-thiadiazolidine-2,5-dithione, 3-phenyl-3;3-phenylbismuthol;5-mercapto-3-phenyl-2h-1,3,4-thiadiazole-2-thione;3,4-Thiadiazolidine-2,5-dithione, 3-phenyl-1
分子式: C8H6N2S3
中文名称: 铋试剂II;3-苯基-1,3,4-噻二唑亚基-2,5-二硫酮;5-硫基-3-苯基-2-硫-1,3,4-硫代二唑,钾盐;铋试剂 II
英文名称: BismuthiolII;5-Mercapto-3-phenyl-2-thio-1,3,4-thiodiazole, potassium salt;4-thiadiazole-2-thione, 5-mercapto-3-phenyl-2h-3;4-thiadiazolidine-2,5-dithione, 3-phenyl-3;3-phenylbismuthol;5-mercapto-3-phenyl-2h-1,3,4-thiadiazole-2-thione;3,4-Thiadiazolidine-2,5-dithione, 3-phenyl-1
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条