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1)  typespecific Indirect ELISA
型特异性间接酶联免疫吸附试验
2)  ELISA [英][i'laizə]  [美][ɪ'laɪzə]
间接酶联免疫吸附试验
1.
The positive clones were picked out and identified by ELISA and WB.
方法以中国莱姆病螺旋体伽氏疏螺旋体(Borreliagarinii)的代表菌株PD91的全菌蛋白为抗原,免疫BALB/c小鼠,取脾细胞与骨髓瘤细胞SP2/0融合,用间接酶联免疫吸附试验(ELISA)和蛋白免疫印迹方法(WB)筛选,并经过2次或3次克隆,以获得单克隆抗体。
2.
Methods Serum IgM antibodies of influenza virus A and B(FluA and FluB),respiratory syncytial virus(RSV),adenovirus(ADV)and parainfluenza virus(PIV)were detected by indirect enzyme linked immunosorbent assay(ELISA).
方法采用间接酶联免疫吸附试验(ELISA)方法检测血清流感病毒A(FluA)、流感病毒B(FluB)、呼吸道合胞病毒(RSV)、腺病毒(ADV)和副流感病毒(PIV)的IgM抗体。
3.
leprae and their trends in sera from NC (192 cases) and CLP (666 cases, including 8 cases relapsed) in ELISAs using ND, Ms, LAM B and α 2 as antigens were mearsured.
方法 : 用间接酶联免疫吸附试验(ELISA)检测正常人 (192例 )和愈后原麻风病人 (6 6 6例 ,含 8例复发者 )血中抗体水平及其态势 ,计算各抗原检测结果间的个体符合率并加以比较。
3)  Indirect enzyme linked immunosorbent assay
间接酶联免疫吸附试验
4)  Indirect ELISA
间接酶联免疫吸附试验
1.
Comparative Studies on Three Different Indirect ELISA Methods With Different Principles in the Diagnosis of SARS;
三种不同原理的间接酶联免疫吸附试验方法在严重急性呼吸道综合征诊断中的比较研究
2.
Antiserum specific for the Vibrio parahaemolyticus was produced from rabbits and used in an indirect ELISA incorporating Alkaline Phosphatase conjugated anti rabbit IgG goat serum, and p Nitrophenyplosphate (pNPP) as substrate.
研究建立中国对虾病原菌┐副溶血弧菌(Vibrioparahaemolyticus)的间接酶联免疫吸附试验(ELISA)快速检测技术。
3.
Establishment of Indirect ELISA Method of Anti-idiotypic Antibodies of MDV;
方法采集琼扩检出MDV羽囊阳性鸡血清,提纯制备IgG抗原和用MDV制备的抗原,免疫接种6~8周龄BALB/c小鼠,不同时期采集血样,用间接酶联免疫吸附试验和竞争抑制试验方法进行动态血清学观测。
5)  enzyme linked immunoadsorbent assay
酶联免疫吸附性试验
6)  indirect competitive inhibition enzyme-linked immunosorbent assay
间接竞争抑制酶联免疫吸附试验
1.
The concentrations of tetrodotoxin in 17 livers and 20 muscles of puffer fishes were detected by mouse bioassay and indirect competitive inhibition enzyme-linked immunosorbent assay (ELISA) respectively.
应用小鼠生物试验和间接竞争抑制酶联免疫吸附试验(ELISA)同步检测17份河豚鱼肝组织和20份河豚鱼肌 肉组织中的河豚毒素含量。
补充资料:酶联免疫吸附试验


酶联免疫吸附试验


酶联免疫吸附试验是将可溶性抗原或抗体吸附(包被)于固相载体(目前常用微孔反应板)上,加入待检样品,所测未知抗体或抗原与包被的抗原或抗体结合,形成抗原抗体复合物,待依序加入酶结合物(酶标记抗体或酶标记抗原)和该酶的底物时,产生呈色反应。呈色程度与检样中待测抗体或抗原的量相关,可据此定性或定量。用于检测精液或宫颈黏液中的抗精子抗体,并行定量分析。方法是以精子或精子抗原提取物放入微试管,加入被测精液或宫颈黏液及抗免疫球蛋白—酶结合物,再加入酶底物观察颜色变化,以自动计算器进行定量测定。
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