2) high conservative region
高保守区
1.
Total RNA was extracted from HAV, and select its high conservative region as goal region, and design a double primers to amplify its nucleotide sequences by RT-PCR, the amplified product was detected with agarose gel electrophoresis, and the goal fragment was observed at 200 bp under UV lamp, then titer of HAV was counted.
提取HAV总RNA,选用HAV高保守区为目标区,设计合成一对引物,通过RT-PCR反应扩增其核酸,用琼脂糖凝胶电泳法检测其扩增产物,紫外灯下观察,约200bp处为目标片段,根据观察结果,计算HAV滴度。
4) CDPK-homologous sequence
CDPK保守区
5) Conservative region of CP
CP保守区
6) DM-box
DM保守区
1.
The 140bp DM-box of AsDMRT1 gene in Alligator Sinensis was amplified by using degenerated PCR, and then subcloned and sequenced.
本文参照人DMRT1基因DM保守区及有关文献,设计了一对简并引物,扩增了扬子鳄的DMRT1基因,并对扩增产物进行了亚克隆与测序。
补充资料：保守复制
分子式：
CAS号：
性质：又称保留复制。一种双链DNA复制的模型。复制模型中亲代的双链并不分离，复制后的子代含原亲代的双倍体及新合成的双倍体。
CAS号：
性质：又称保留复制。一种双链DNA复制的模型。复制模型中亲代的双链并不分离，复制后的子代含原亲代的双倍体及新合成的双倍体。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
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